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1	Academic Health System	ABS-63
Nurse Motivation Associated with Caring Behavior of Nurses in the Inpatient Room of Indramayu Hospital Wiwin Nur Aeni (a*), Cynthia Hardivianty (a), Lukman Nul Hakim (a) a) Indramayu College of Health Science Wirapati Street, Indramayu Abstract Background: The implementation of caring behavior at Indramayu Regional Hospital has only reached 53.1%. The lack of caring behavior in nursing services can result in the quality of nursing services, which can lead to decreased patient satisfaction and increased days of hospitalization. While providing care, nurses need to have high motivation in carrying out nursing care services. This study aims to determine the relationship between nurses motivation and caring behavior in the Inpatient Room of Indramayu Hospital. Methods: The research method used was analytic correlation with a cross sectional approach. The population were all executive nurses in seven inpatient rooms at Indramayu Hospital. The sampling technique used was total sampling with a total of 91 respondents. Data collection was done using a questionnaire. Data analysis used was univariate and bivariate with chi-square test. Results: The results of the study 82 (90.1%) respondents had high motivation and 84 (92.3%) respondents applied caring behavior well. The results of bivariate analysis for motivation and caring behavior of nurses obtained a p value of 0.000 (&#945- < 0.05).. Conclusion: The conclusion of the study is there is a relationship between nurse motivation and nurse caring behavior. It is recommended for nurses to maintain motivation in providing nursing care. So that it can improve caring behavior. Keywords: Caring- Motivation- Nurse^s Share Link | Plain Format | Corresponding Author (Wiwin Nur Aeni)

2	Academic Health System	ABS-64
Anxiety level on the Medical Student in Bandung Kyla Rucira, Respati Titik, Nurhayati Eka Universitas Islam Bandung Abstract Mental health is an essential factor in overall health, especially for medical students who are prone to anxiety. Anxiety can negatively impact daily functioning of the medical students, which is necessary for optimal college work. This study analyzes the anxiety levels in Medical Faculty students in Bandung. This descriptive research method was conducted on medical faculty college students in October 2024-samples were collected by stratified random sampling with 105 respondents. Data were collected through the Taylor Manifest Anxiety Scale (TMAS) questionnaire to measure anxiety levels, data analysis was carried out using the help of Microsoft Excel based on characteristic and anxiety levels. The characteristic of the respondents based on gender are mostly female (60%), with the majority of age 20 (33,33%) in range 18-23 years of age. For anxiety, the research result showed that most respondents were included in the mild category, namely 38 people (36.19%), followed by the severe category, namely 35 people (33.33%). The moderate category consists of 31 people or (29.51%), with one person (0.95%) is considered in nonanxious ones. This study concludes that most of the students experience anxiety with different variety, which may affect the mental health of the students and can interfere their college life. Keywords: anxiety, medical student, mental health Share Link | Plain Format | Corresponding Author (Rucira Kyla Rubiati)

3	Biochemistry	ABS-52
Separation of Cephalosporin-C Acylase from Fermentation Broth with Polyethylene Glycol-Salt Aqueous Two-Phase Systems Sasmito Wulyoadi1, Nabilla Amara Denaputri2, Ahmad Wibisana1 1Research Center for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency of Indonesia, Cibinong, Bogor, Indonesia 2Biotechnology Study Program, Faculty of Science and Mathematics, Diponegoro University, Semarang, Indonesia Abstract This study investigates the separation of cephalosporin-C acylase (CCA) using an Aqueous Two-Phase System (ATPS) composed of polyethylene glycols (PEGs) and salts. CCA is an enzyme essential for the one-step bioconversion of cephalosporin-C into 7-amino cephalosporanic acid (7-ACA), a key intermediate for synthesizing broad-spectrum, semi-synthetic cephalosporin antibiotics. ATPS, a separation technique based on immiscible liquid phases-typically formed by two polymers or a polymer and a salt-offers advantages over conventional extraction methods, including simplicity, environmental friendliness, rapid separation with minimal enzyme denaturation, high selectivity and scalability. While ATPS has been widely applied to other enzymes like penicillin acylase, amylase, protease and pectinase, no studies, to our knowledge, have reported on CCA separation using this method. Our study utilizes PEGs (400, 2000, 4000, 6000 and 10000) and salts (sodium sulfate, ammonium sulfate, sodium citrate and dipotassium hydrogen phosphate) to explore optimal ATPS conditions for CCA separation. Two critical factors in evaluating ATPS efficiency for CCA were yield (recovery percentage) and purification factor (purity relative to crude extract). The separation process involved CCA fermentation with Escherichia coli, cell lysis, and phase formation using PEG and salt concentration variations in a phosphate buffer (0.1 M, pH 8.5). Results showed that the highest CCA recovery was achieved with 21% PEG 2000, 5% K2HPO4, and 74% phosphate buffer, yielding 83.0% in the top phase with a purification factor of 1.80 relative to the crude extract. Keywords: Cephalosporin-C Acylase, Aqueous Two-Phase System, Polyethylene Glycol, Salts, Enzyme Recovery, Purification Factor Share Link | Plain Format | Corresponding Author (Sasmito Wulyoadi)

4	Biochemistry	ABS-71
Enhancing the potency of kappa-carrageenan for its biomedical applications Amalina Ghaisani Komarudin (a), Azis Adharis (b), Agustiningsih (a), Suharti (b) (a) Eijkman Research Center for Molecular Biology, Research Organization for Health, National Research and Innovation Agency (b) Chemistry Study Program, Faculty of Science and Computer Science, Universitas Pertamina Abstract Seaweed, an abundant natural resource in Indonesia, is a rich source of carrageenan, a complex sulfated polysaccharide with diverse biological activities. However, its high molecular weight contributes to significant viscosity and low solubility, limiting its therapeutic potential. The antiviral and anticancer properties of kappa-carrageenan oligosaccharides and their sulfated derivatives remain largely underexplored. This study aims to depolymerize kappa-carrageenan, perform sulfation, and evaluate the cytotoxicity of the resulting compounds using cell lines commonly employed in viral infection studies. Kappa-carrageenan was depolymerized via microwave-assisted hydrolysis with hydrochloric acid, followed by sulfation using chlorosulfonic acid. The resulting oligosaccharides and their sulfated derivatives were characterized using Fourier Transform Infrared (FTIR) Spectroscopy, while molecular weight was determined through the glucose microdetermination method. Depolymerization yielded kappa-carrageenan oligosaccharides with a molecular weight of approximately 2 kDa. Cytotoxicity was assessed via the MTT assay, a colorimetric method for evaluating cell viability. The CC50 values for kappa-carrageenan oligosaccharides and their sulfated derivatives in A549, HEp-2, Vero E6, and RD cell lines exhibited a consistent trend, with CC50 values around 0.8 mg/mL for the oligosaccharides and exceeding 4 mg/mL for the sulfated derivatives. These findings underscore the promising biomedical potential of kappa-carrageenan oligosaccharides and their sulfated derivatives as candidates for antiviral activity evaluation in vitro. (Word count: 212) Keywords: carrageenan, kappa carrageenan oligosaccharides, depolymerization, cytotoxixity assay Share Link | Plain Format | Corresponding Author (Amalina Komarudin)

5	Degenerative Disease	ABS-12
Quantitative Measurement of Langerhans Islet Impairment of Diabetic Rat Model With Cigarete Exposure Yuktiana Kharisma,1 Yuke Andrianne,2 Abdul Hadi Hassan,1 Ratih Syifa H3 1 Anatomic Pathology Department, Medical Faculty oF Universitas Islam Bandung, Indonesia 2 Pharmacology Department, Medical Faculty oF Universitas Islam Bandung, Indonesia 3 Undergraduate Program, Medical Faculty oF Universitas Islam Bandung, Indonesia Abstract Diabetes mellitus and cigarette smoke exposure are dominant health issue around the world. These conditions affect the organs, especially pancreas. This study aims to evaluate the impairment of Langerhans islet in diabetic rats model exposed to cigarette smoke. This study evaluates the density, area, and cell count within Langerhans islet diabetic rats exposed to cigarette smoke. A total of 18 rats were divided into 3 groups (n=6 per group). Group 1 was healthy rats (control), group 2 was diabetic rats exposed to cigarette smoke, and group 3 was treated with glibenclamide and simvastatin. Pancreatic tissue was collected and stained using Hematoxylin-Eosin (HE) with 100x, 400x magnification. Data normality was assessed using the Shapiro-Wilk test. One-Way ANOVA was used to compare the density and cell count, while the Kruskal-Wallis test was applied to Langerhans islet area. Significant differences in islet density were identified (p< 0.01). However, no significant differences were observed in area and cell count across groups (p=0.33) and (p=0.22). Bonferroni post hoc test revealed significant differences between groups 1 and 2 (p=0.002), groups 1 and 3 (p=0.003), but no significant difference between groups 2 and 3 (p=1.000). Diabetic with cigarrete exposure impact significantly the quantitative measurement of Langerhans islet especially the density of Langerhans islet. Diabetic and cigarrete induces exacerbating cellular damage by increased reactive oxygen species production, contributing to impairement of pancreatic cell. Simvastatin and glibenclamide appear to mitigate these adverse effects, suggesting their potential utility in managing diabetes complicated by smoking. Keywords: Diabetic, Langerhans Cell, Pancreas, Smoking Share Link | Plain Format | Corresponding Author (Yuktiana Kharisma)

6	Degenerative Disease	ABS-18
Multimorbidity in Coronary Artery Diseases Patient Setyo Wahju Wibowo, Wida Purbaningsih, Yani Triyani. Faculty of Medicine UPI, Faculty Of Medicine Unisba. Abstract Multimorbidity, the coexistence of multiple chronic conditions, is a growing concern in patients with coronary artery diseases (CAD). This study aims to analyze the characteristics and outcomes of CAD patients with cardiovascular (CV) and non-cardiovascular (non-CV) multimorbidity. We conducted a cross-sectional analysis study of 371 CAD patients, categorizing them into four groups: no multimorbidity, CV multimorbidity, non-CV multimorbidity, and both CV and non-CV multimorbidity. Demographic data and outcomes were compared across these groups. Among the patients, 44.5% had no multimorbidity, 17.3% had CV multimorbidity, 3.6% had non-CV multimorbidity, and 29.0% had both CV and non-CV multimorbidity. The age distribution showed a higher prevalence of multimorbidity in older patients. Males constituted 60.3% of the CV multimorbidity group, while females were more prevalent in the non-CV multimorbidity group (51.4%). The outcome analysis revealed that the presence of multimorbidity significantly impacted patient survival, with higher mortality rates observed in patients with both CV and non-CV multimorbidity. The study highlights the significant burden of multimorbidity in CAD patients and its association with adverse outcomes. These findings underscore the need for comprehensive management strategies to address the complex healthcare needs of this population. Keywords: Coronary artery disease, multimobidity Share Link | Plain Format | Corresponding Author (Wida Purbaningsih)

7	Degenerative Disease	ABS-20
The Association Between TILs and Cancer Grading in Breast Cancer Patients at Cibabat Hospital in 2022-2023 Meike Rachmawati,1 Ismet Muchtar Nur,1 Muhammad Syah Misuari Sabirin,1 Rian Robian,2 Adhi Nugraha,2 Rina Melati,3 Aryanti,3 Aninditya Putri Anugrah,4 Nazmy Noor Laila,4 Reyiena Kusumaryani Rahmat,4 Afni Nur Hasanah,4 Nuzirwan Acang,5,6 Wida Purbaningsih,7 1 Department of Anatomy Pathology, Faculty of Medicine, Universitas Islam Bandung 2 Surgical Oncology Installation, Cibabat Regional General Hospital, Cimahi, Indonesia 3 Laboratory of Anatomy Pathology, Al Islam Hospital, Bandung, Indonesia 4 Biopath Laboratory, Bandung, Indonesia 5 Department of Internal Medicine, Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia, 6 Internal Medicine Installation, Cibabat Regional General Hospital, Cimahi, Indonesia 7 Department of Histology, Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia Abstract Breast cancer is the most prevalent malignancy in women globally (154 out of 185 countries) and the world^s fifth-most frequent cause of cancer death. Breast cancer grading and TILs are prognostic factors that can be explained by histology. The purpose of this study is to examine the association between TILs and breast cancer grade in breast cancer patients at Cibabat Hospital. The method used in this research is descriptive-analytic with a cross-sectional design using hematoxylin-eosin slide preparations of breast cancer patients at Cibabat Hospital from 2022 to 2023 who met the inclusion criteria. The results showed that the majority of breast cancer patients at Cibabat Hospital had grade 3 (34.9%) and low TILs (65.1%). Statistical analysis showed no significant difference between TILs and breast cancer grade (p = 0.864- 0.864> 0.05). The conclusion of this study is that there is no association between TILs and histological grade of breast cancer in breast cancer patients at Cibabat Hospital. Keywords: Breast cancer, Histological grading, TILs Tumour-Infiltrating Lymphocytes (TILs) Share Link | Plain Format | Corresponding Author (Meike Rachmawati)

8	Degenerative Disease	ABS-22
Metabolic Disease Screening In Women At Posyandu Sejahtera As One Of The Preventive Efforts To Prevent Breast Cancer Meike Rachmawati,1, a) Mira Dyani Dewi,2 Muhammad Syah Misuari Sabirin,1 Reyiena Kusumaryani Rahmat,3 Aninditya Putri Anugrah,3 Nazmy Noor Laila,3 Afni Nur Hasanah3 1Department of Anatomy Pathology, Faculty of Medicine, Universitas Islam Bandung 2Department of Obstetrics and Gynecology, Faculty of Medicine Universitas Islam Bandung 3Biopath Laboratory, Bandung, Indonesia Abstract The incidence and mortality rate of breast cancer in the world rank first. The risk factors for this malignancy are closely related to lifestyle changes. Based on the relationship between unhealthy lifestyles and the causes of breast cancer, especially Posyandu Sejahtera women who do not understand the importance of a healthy lifestyle, it is necessary to hold community service that can provide an understanding of this. The activity was attended by 30 Posyandu Sejahtera women from Ciroyom Village, Bandung. The method used was by providing counseling on metabolic disease screening for Posyandu Sejahtera women as one of the preventive efforts for breast cancer prevention. The results of the activity evaluation showed that most participants had BMI with obesity results, normal blood sugar, normal uric acid, normal total cholesterol, high blood pressure, very high body fat, and normal visceral fat. The conclusion of this community service activity is that almost all of the examination results on the women at Posyandu Sejahtera showed normal results, except for BMI, body fat, and blood pressure, which are risk factors for breast cancer. Keywords: Breast cancer, Metabolic disease screening Share Link | Plain Format | Corresponding Author (Meike Rachmawati)

9	Degenerative Disease	ABS-26
Relationship Between Hematocrit and HbA1C as Indicators of Glycemic Control in Diabetes Mellitus Yuke Andriane, Sara Puspita, Ratna Damailia, Fajar Awalia Yulianto Faculty of Medicine Universitas Islam Bandung Abstract Diabetes mellitus (DM) is a chronic metabolic disorder characterized by elevated blood sugar levels that can damage various organs, including the heart, blood vessels, kidneys, and nerves. Diagnosing DM typically involves assessing blood glucose levels and hemoglobin A1c (HbA1c), which reflects average glucose levels over the past 2 to 3 months. HbA1c testing has limitations, particularly due to high costs and availability issues in some laboratories in Indonesia. Hematology parameters are crucial in diagnosing patients. Slower glycosylation and elevated HbA1c levels can lead to functional and structural changes in hemoglobin, increasing erythrocyte viscosity and leading to cell osmotic disturbances. Specific hematological parameters may indicate inflammation associated with DM. The research aims to analyze the correlation between hematocrit and HbA1C to identify alternative tests for predicting glycemic status in resource-limited healthcare settings. It analyzes the correlation between hematocrit and HbA1c as indicators of glycemic status from 603 type 2 diabetes patients. The results indicate that hematocrit has a positive and statistically significant correlation with HbA1c (positive linear regression coefficient), although the correlation is very small. An increase of 1 unit of hematocrit is associated with a rise of 0,03 units of HbA1c. This finding suggests that hematocrit can be used to predict glycemic status in patients with DM. Healthcare providers in facilities with limited resources can utilize this information to improve patient care. Keywords: Key words: type 2 DM, HbA1c, hematological parameters, hematocrit Share Link | Plain Format | Corresponding Author (Yuke Andriane)

10	Degenerative Disease	ABS-27
Correlation of Anthropometric Measurements with Body Mass Index among Premenopausal Women Residing in The Tamansari Health Center Area Ismawati(1*), Sara Puspita (1), Ivania Yuliza (1), Regina Cintya (1), Fitri Rahmawati (1) 1) Faculty of Medicine Universitas Islam Bandung Abstract Premenopause in women is marked by a decrease in estrogen levels before menopause, which can contribute to fat accumulation, particularly in the abdominal area. This often leads to weight gain, resulting in an increased body mass index (BMI). While BMI is a commonly used measure to assess obesity, waist circumference and body fat percentage are less frequently employed, even though they may better correlate with visceral fat levels. This study focuses on examining the relationships between BMI, body fat percentage and waist circumference in premenopausal women residing in the Tamansari Health Center area of Bandung City. Anthropometric data collection involved measuring height, weight, and waist circumference. BMI was calculated accordingly, and body fat percentage was assessed with the Karada Scan tool. The research design utilized an associative, cross sectional approach, sampling 57 premenopausal women over 40 years old who had no prior diagnoses related to metabolic syndrome and had not undergone treatment. The statistical analysis applied was the Pearson Correlation Test. Findings revealed an average age of 47.5 (+- 3.9) years and an average BMI of 26.98 (+- 4.37). The analysis indicated a very strong correlation between BMI and body fat percentage r (57) = 0.83, p < 0.05 and between BMI and waist circumference r (57) = 0.81, p < 0.05. In conclusion, the study established a strong correlation between BMI and both body fat percentage and waist circumference. It suggests that any increase in BMI among premenopausal women is likely accompanied by increases in both body fat percentage and waist circumference. Keywords: anthropometric measurement, BMI, premenopause Share Link | Plain Format | Corresponding Author (Ismawati Ismawati)

11	Degenerative Disease	ABS-42
CHOLESTEROL SCREENING FOR ELDERLY CONGREGANTS OF DKM NURUL ISLAM, CAMPAKA VILLAGE, AS AN EARLY DETECTION EFFORT FOR NON-COMMUNICABLE DISEASE Ariko Rahmat Putra, Ninuk Permata Sari, Alvira Widiyanti, Ajeng Kartika Sari Universitas Islam Bandung Abstract Non-Communicable Diseases (NCDs) in the elderly encompass various health conditions commonly associated with aging. These diseases often develop gradually and are typically asymptomatic in their early stages, making early detection crucial. The Nurul Islam Mosque Welfare Council (DKM) in Campaka Village, Andir District, primarily serves a congregation consisting of elderly individuals. Based on brief interviews, many elderly congregants reported experiencing age-related health issues. This study is descriptive in nature. The population and sample consisted of all elderly congregants of DKM Miftahul Jannah. The study aimed to describe the results of a simple cholesterol screening conducted among the congregants as an effort for early detection of non-communicable diseases. The research utilized the Point-of-Care Testing (POCT) method. The findings showed that out of 64 participants (14 men and 50 women), 34% had total cholesterol levels of <200 mg/dL, while 66% had levels >200 mg/dL. This study only conducted a simple screening for total cholesterol levels. To further assess the risk of non-communicable diseases, more comprehensive tests, such as measurements of triglycerides, HDL, and LDL levels, are needed. Keywords: Cholesterol Screening, Elderly Congregants, Non-Communicable Disease Share Link | Plain Format | Corresponding Author (Ariko Rahmat Putra)

12	Degenerative Disease	ABS-48
The Relationship Between Stroke Lesion Location and Cognitive Function Impairment in Ischemic Stroke Patients Zahra Khairunnisa Karimah1, a), Fajar Handika1, b), Poca Anida1, c), Mochammad Faisal Afif Mochyadin2, d), Nugraha Sutadipura3, e), Alya Tursina2, f) 1Undergraduate Program, Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia 2Department of Neurology, Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia 3Department of Biochemistry, Nutrition, and Biomolecular, Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia Abstract Stroke is the leading cause of death in Indonesia, accounting for 19.42% of all fatalities. According to data from the Basic Health Research (Riskesdas), the incidence of stroke in Indonesia increased by 56% from 2013 to 2018. In Indonesia, ischemic stroke accounts for a substantial 67% of all stroke cases. Poststroke cognitive impairment significantly contribute to morbidity and mortality after a stroke. There is a strong association between location of stroke lesions and cognitive function impairment. The purpose of this study was to study the relationship between the location of supratentorial, infratentorial and both ischemic stroke lesions with the MoCA-Ina score. This study was an observational study with a cross sectional study design. The study was conducted on 47 patients who were first diagnosed with a stroke ischemic from a head CT scan results in the Neurology Department of RSAU Dr. M. Salamun Bandung in August to October 2024. The research instrument is MoCA-Ina questionnaire. Version 17 of the social sciences statistics (SPSS) was used to process data. The results showed that among ischemic stroke patients, 31 out of 35 with supratentorial lesions (88.5%) experienced cognitive function impairment. Additionally, 9 out of 11 patients with both supratentorial and infratentorial lesions (81.8%) experienced cognitive function impairment. Notably, no patients with only infratentorial lesions experienced cognitive function impairment. The chi-square analysis of the data yielded a p-value of 0.046, indicating a significant relationship (p < 0.05). In conclusion, there was a relationship between stroke lesions location and cognitive function impairment in ischemic stroke patients. These findings highlight the importance of early detection and targeted interventions to manage cognitive impairment in stroke patients. Keywords: Ischemic stroke, location of lesions stroke, cognitive function impairment Share Link | Plain Format | Corresponding Author (Zahra Khairunnisa Karimah)

13	Degenerative Disease	ABS-53
The Relationship Between Lipid Profile and Cognitive Function in Ischemic Stroke Patients Fajar Handika(a), Zahra Khairunnisa Karimah(a), Poca Anida(a), Clarissa Ratna Shamira Hakriadi(a), Mochammad Faisal Afif Mochyadin(b), Alya Tursina(b) a) Undergraduate Program, Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia b) Department of Neurology, Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia Abstract Stroke is the second leading cause of death globally, with ischemic stroke accounting for over 62% of cases and cognitive impairment as a frequent complication. Modifiable risk factors like diabetes, hypertension, and LDL cholesterol levels are linked to cognitive decline, with dyslipidemia recognized as a significant risk factor. High serum cholesterol, LDL, and non-HDL cholesterol levels contribute to cognitive impairment due to their atherogenic effects, and studies indicate a correlation between elevated triglyceride and LDL levels and reduced memory abilities. This study aims to analyze and test the hypothesis that there is an association between lipid profiles in this case in ischemic stroke patients with dyslipidemia and hyperlipidemia with cognitive function in ischemic stroke patients. This cross-sectional study involved 55 ischemic stroke patients. Cognitive function was assessed using the MoCA-INA instrument, while lipid profile data were obtained from medical records. Results showed that 70.9% of patients had dyslipidemia, and 29.1% had hyperlipidemia. Among these, 65% of patients with dyslipidemia experienced cognitive impairment, compared to 20% of those with hyperlipidemia. Statistical analysis using the Chi-Square test revealed a significant relationship between lipid profiles and cognitive function in ischemic stroke patients (p=0.024). Discussion highlights that dyslipidemia affects cognitive function through mechanisms such as atherosclerosis and impaired cerebral blood flow. Abnormal lipid profiles, especially elevated LDL and triglycerides, contribute to cognitive decline by exacerbating vascular dysfunction and increasing blood-brain barrier permeability. These findings underscore the importance of lipid profile management, including lifestyle modifications, pharmacological therapy, and dietary interventions, to prevent further cognitive decline in ischemic stroke patients. Keywords: Lipid profile, dyslipidemia, hyperlipidemia, cognitive function, ischemic stroke, MoCA-INA Share Link | Plain Format | Corresponding Author (Fajar Handika)

14	Genetic and Molecular Biology	ABS-41
Acute and sub-chronic oral toxicity study of purple sweet potato (Ipomoea batatas [L.] Lam) yogurt in mice (Mus musculus) Astrid Feinisa Khairani (a), Yunisa Pamela (a), Nandina Oktavia (a*), Achadiyani Achadiyani (a), M. Yusuf Adipraja (b), Prita Yasri Zhafira (b), Widad Aghnia Shalannandia (a), Nur Atik a. Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, West Java, Indonesia- b. Undergraduate Program, Faculty of Medicine, Universitas Padjadjaran, West Java, Indonesia. Abstract The purple sweet potato yogurt (PSPY) product was developed to combine the benefits of probiotic activities in yogurt and the bioactive effects of anthocyanin in purple sweet potato. Aspect of food safety important to be evaluated in preventing any potentially harmful side effects of food product. This study aims to investigate acute and sub-chronic oral toxicity of PSPY in mice. Methods used to evaluate acute oral toxicity was by a 14-days observation for any clinical sign of toxicity on fifteen female balb/c mice following a single dosage of PSPY (nil, 2 or 5 g/kg body weight), whilst for the sub-chronic oral toxicity was conducted to four groups of mice with the dose of 0, 12, 20, and 40 g/kg body weight for 28 days, and another group of mice with 40 g/kg body weight for 14 days longer to observe any delayed toxicity effect. Daily observation was made on body weight and clinical signs of toxicity. Study about the liver and kidney macroscopy, microscopy and relative organ weight, liver enzyme, and haematology profile analyses were done at the end of the study. Results found there were no signs of acute toxicity observed and no abnormality in body weight, relative organ weight, and gross organ examination. In the sub-chronic toxicity found no clinical signs of toxicity, no significant differences in body weight, relative liver weight, liver enzymes, haematology profile, or abnormality in gross and histological examination of the liver. Conclusion of study found that oral administration of PSPY in mice did not result in acute toxicity, while the dosage up to 40 g/kg body weight did not lead to sub-chronic toxicity. Keywords: acute oral toxicity, anthocyanin, purple sweet potato, sub-chronic oral toxicity, yogurt Share Link | Plain Format | Corresponding Author (Nandina Oktavia)

15	Genetic and Molecular Biology	ABS-47
Microglandular Hyperplasia of the Cervix: A Case Report and Literature Review Muhammad Syah Misuari Sabirin (a*,b), Meike Rachmawati (a,b), Ismet Muchtar Nur (a), Abdul Hadi Hassan (a), Yuktiana Kharisma (a), Meta Maulida (a), Ermina Widyastuti (a), Afiati (b) a) Faculty of Medicine, Universitas Islam Bandung. Jalan Tamansari No. 20, Bandung 40116, Indonesia *sabslab.ms[at]gmail.com b) Laboratorium Biopath. Jalan Jend. Sudriman No. 655 A, Bandung 40164, Indonesia Abstract Microglandular hyperplasia (MGH) is a rare, benign non-neoplastic glandular proliferation of the endocervix that often found incidentally in reproductive women. MGH usually can be found in a patient with abnormal uterine bleeding or hormonal imbalance. This case report describes a 21-year-old woman who presented with solitary, polypoid mass from uterine cervix. Histopathological examination of a cervical biopsy revealed features characterized by the presence of numerous small, crowded, complex proliferation of glands with minimal cytological atypia and increased glandular to stromal ratio. These features are consistent with MGH. The patient had no significant medical history, and her clinical presentation suggested no underlying hormonal imbalance. A single excision is performed with no followed conservative therapy. This case highlights the importance of considering MGH in young women presenting with polypoid lesion from cervix and emphasizes the role of histopathological examination in distinguishing this condition from malignant variants. The management approach in this patient provided a satisfactory clinical outcome. Keywords: Case report, cervix, histopathology, microglandular hyperplasia Share Link | Plain Format | Corresponding Author (Muhammad Syah Misuari Sabirin)

16	Genetic and Molecular Biology	ABS-54
Soyghurt Potentially Controls the Level of sFlt1 and PLGF in Preeclampsia Maternal Serum-Induced Placental Trophoblast Cells in Vitro Astrid Feinisa Khairani1, Uci Ary Lantika2, Julia Ramadhanti1, Muhammad Hasan Bashari1,3, Widad Aghnia Shalannandia4, Tenny Putri Wikayani5, Achadiyani Achadiyani1, Mulyanusa Amarullah Ritonga6 1Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Jatinangor, West Java, Indonesia- 2Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Bandung, Bandung, West Java, Indonesia- 3Research Centre of Oncology and Stem Cell, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia- 4Graduate School of Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Indonesia, 5Culture and Cytogenetic Laboratory, Universitas Padjadjaran, Bandung, West Java, Indonesia- 6Department of Obstetrics and Gynecology, Hasan Sadikin General Hospital, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia Abstract Background: Soyghurt is a symbiotic drink that consists of probiotics as the fermentation agent, which could increase the beneficial effect of soybeans. Preeclampsia is one of the contributors to mortality in pregnant women and their children worldwide. Escalation of inflammation, oxidative stress, and endothelial dysfunction are the aspects that promote an imbalance condition of angiogenic substance (sFLT-1) and antiangiogenic (PLGF) in preeclampsia. One of the prospective ways to prevent preeclampsia is by utilizing functional food such as soyghurt. This study aims to evaluate the effect of soyghurt supernatant on other preeclampsia markers (sFLT-1 and PLGF) levels in the primary placenta culture, which had been induced by preeclampsia serum. Methods: The trophoblast primary culture was induced by preeclampsia serum (10%). The Soyghurt treatment was performed with 2.5%, 5%, and 7.5% Soyghurt supernatant concentrations in culture media. The expression of preeclampsia markers, sFLT-1, and PLGF were evaluated using ELISA. Results: Expression sFLT-1 on preeclampsia-induced cell culture treated with Soyghurt was significantly lowered compared to the untreated group (p<0.01). However, no significant difference was observed in the PLGF levels of all groups induced by preeclampsia serum (p>0.05). Conclusion: This study demonstrates the potential impact of Soyghurt in balancing preeclampsia marker expression by inhibiting the expression of sFLT-1 in preeclampsia serum-induced trophoblast cells. Keywords: preeclampsia, PLGF, sFLT-1, soya yoghurt, trophoblast Share Link | Plain Format | Corresponding Author (Astrid Feinisa Khairani)

17	Genetic and Molecular Biology	ABS-70
Biodiversity of Bacteriophages Specific to ESBL-Producing Klebsiella pneumoniae Isolated from Wastewater Nuridha Audinia Safitri (a,e*), Meity Mardiana (e), Leli Saptawati (c), Dewi Anggraini (d), Iftita Rahmatika (b), Cindy Rianti Priadi (b), Ruby Setiawan (f), Yustinus Maladan (e), Andriansjah Rukmana (a), Dodi Safari (e), Yulia Rosa Saharman (a), Rosantia Sarassari (e) a) Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia *nuridhaaudinias[at]gmail.com b) Environmental Engineering Study Program, Faculty of Engineering, Universitas Indonesia, Jakarta, Indonesia c) Department of Microbiology, Faculty of Medicine, Universitas Sebelas Maret, Surakarta, Indonesia d) Department of Microbiology, Faculty of Medicine, Universitas Riau, Riau, Indonesia e) Eijkman Research Center for Molecular Biology, National Research and Innovation Agency, Bogor, Indonesia f) Biosystematics and Evolution Research Center, National Research and Innovation Agency, Bogor, Indonesia Abstract Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae (ESBL-KP) is an antimicrobial-resistant (AMR) bacteria that poses a major challenge in infection treatment. Bacteriophages are viruses that infect bacteria. Phage plaque morphology can serve as an initial screening to identify lytic or lysogenic bacteriophages that can be potential alternative for antimicrobial teraphy. This study aims to characterize and analyze the diversity of bacteriophages infecting ESBL-KP from wastewater based on plaque morphology. The study was conducted from April to May 2024. Wastewater samples were collected from seven locations. Samples were filtered using a 0.22 Millipore membrane filter. The resulting filtrate was mixed with suspensions of 29 ESBL-KP isolates. Phage plaque morphology was tested using the double overlay plaque assay and spot test. The formed plaques were observed and analyzed based on turbidity and the presence or absence of halos. This study isolated a total 160 bacteriophages from seven wastewater sample points againts 29 ESBL-KP isolates. Of these, 101 phages formed turbid plaques and 59 formed clear plaques. Among the 59 clear plaques, 30 formed with halos (semi-transparent zones around the plaques). Clear plaques indicate lytic (virulent) activity, whereas turbid plaques indicate lysogenic (temperate) activity. This study is an initial investigation into bacteriophages. Morphological analysis reported of diversity among bacteriophages isolated from wastewater. In order to know which phage is suitable for future teraphy, genome analysis, and interaction between host and phages are needed to be explored. Keywords: bacteriophage, Klebsiella pneumoniae, ESBL, wastewater, plaque turbidity Share Link | Plain Format | Corresponding Author (Nuridha Audinia Safitri)

18	Genetic and Molecular Biology	ABS-72
The Conundrum of Lymphocyte and CD4+ Alteration in IGRA Results of Transfusion-dependent Thalassemia After Vitamin D Supplementation: An Open-label, Single-arm Clinical Study Mohammad Ghozali (1)*, Findania Ulfah Afanin Kustandari (2)*, Nur Melani Sari (3), Suhendra Praptama (2), Agnes Rengga Indrati (3), Budi Setiabudiawan (4), Ramdan Panigoro (1) (1) Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Sumedang, West Java, Indonesia. (2) Faculty of Medicine, Universitas Padjadjaran, Sumedang, West Java, Indonesia. (3) Department of Clinical Pathology, Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia. (4) Departement of Pediatrics, Universitas Padjadjaran, Bandung, West Java, Indonesia. Abstract Introduction: Iron overload and anemia in transfusion-dependent thalassemia patients can impair lymphocytes and CD4+ T cell function, increasing infection susceptibility. Immune cells possess vitamin D receptors (VDR) and exhibit immunomodulation- however, the effect of vitamin D supplementation requires additional investigation. Purpose: Assess the impact of vitamin D supplementation on CD4+ T lymphocytes in transfusion-dependent thalassemia patients by comparing interferon-gamma release assay (IGRA) results. Methods: A non-randomized, open-label trial was performed on pediatric beta-thalassemia major patients undergoing regular blood transfusions and latent tuberculosis screening using QFT-GIT at Hasan Sadikin General Hospital, Bandung, Indonesia. Participants received 800 IU/day or 2000 IU/day of cholecalciferol based on their vitamin D status. After 8 weeks, various parameters, including iron status, vitamin D levels, hematological profile, IGRA, CD4+ T cell count and proportion, and lymphocyte levels were assessed. Transfusion profiles, erythrocyte indices, iron status, and 25(OH)D levels between groups were compared using unpaired student t-tests, while efficacy endpoints were analyzed with paired student t-tests. Results: Fifty-seven iron overload patients showed a significant increase in vitamin D levels after supplementation (9.45 ng/dL (6.93-15.77) to 10.95 ng/dL (8.31-19.81)- P=0.003). The QFT-GIT indicated 33 participants (26%) in the indeterminate group. All subjects exhibited a significantly low CD4+ T cell count post-supplementation, with higher CD4+ T cell count, lymphocyte count, and lymphocyte proportion observed in the determinate group. Conclusion: Low-dose vitamin D supplementation modulated CD4+ T cell counts and increased lymphocyte numbers, particularly in immunocompetent transfusion-dependent thalassemia. Keywords: Vitamin D, CD4+ T Cell, Thalassemia, Interferon-gamma Share Link | Plain Format | Corresponding Author (Mohammad Ghozali)

19	Genetic and Molecular Biology	ABS-74
Does CCL4 Effective to Induce Acute Kidney Injury in Wistar Rats? Tita Barriah Siddiq1, a), Meta Maulida Damayanti2, b), Ismet Muchtar Nur2, c), Ratu Adeline3, 1Department of Medical Education, Bioethics and Humanity, Faculty of Medicine, Universitas Islam Bandung, Tamansari Bandung, West Java, Indonesia 2Department of Pathology Anatomy, Faculty of Medicine, Universitas Islam Bandung, Tamansari Bandung, West Java, Indonesia 3Universitas Islam Bandung, Tamansari Bandung, West Java, Indonesia Abstract Acute kidney injury (AKI) is described as the sudden loss of renal excretory function. AKI is one of a group of diseases characterized as acute kidney diseases and disorders, in which progressive decline in kidney function or persistent kidney dysfunction is associated with irreversible loss of kidney cells and nephrons. Nephrotoxicity induced by chemicals and drugs is the main cause of acute kidney injury. Carbon tetrachloride (CCl4) is the most potent toxin, and is widely used in scientific studies to create experimental models that simulate oxidative stress in various pathophysiological scenarios. The purpose of this study was to determine whether CCL4 can effectively cause kidney injury in rats. This study was a true experimental study using eighteen adults male Wistar rats weighing between 200 and 300 g, which were induced by CCL4 for kidney injury. Kidney function tests and histopathological image analysis were performed. The results showed that there was an increase in Blood Urea Nitrogen (BUN) and serum creatinine levels in the treatment group compared to the control group with p <0.05, and histopathological found kidney damage in the form of atrophic tubules with necrotic lining cells. It can be concluded that CCL4 is a fairly strong nephrotoxic material for kidney injury that causes oxidative stress by reducing the activity of antioxidant enzymes, the release of inflammatory cytokines, and apoptosis in the treatment of nephrotoxicity due to drugs or chemicals. Keywords: Acute kidney injury- Animal model- CCL4 Share Link | Plain Format | Corresponding Author (Ratu Adeline)

20	Herbal Medicine	ABS-10
Exploring the Potential of Plants as Medical Raw Material Maya Tejasari (a), Listya Hanum Siswanti (a*) a) Histology Department, Bandung Islamic University, Jalan Tamansari No.22, Bandung, Indonesia *listya[at]unisba.ac.id Abstract Prolonged hyperglycemia can trigger tissue damage due to the formation of free radicals that affect vital organs, including the brain. Medicinal plant derivatives with hypoglycemic properties have been used in traditional medicine and traditional healing systems, i.e. kersen leaves (Muntingia calabura L.). This study aims to investigate the protective effects of water-extracted kersen leaves (Mutingia calabura L.) on brain histopathology in mice (Mus musculus) induced with alloxan, a compound known to cause hyperglycemia. This is an invivo study, used a total of 30 mice which were divided into 5 groups and given alloxan induction with a dose = 0.442 mg / 20gBW. The dose of cherry leaf extract was 200, 400, and 800 mg / grBW which was given once a day for 14 days. According to the study, giving kersen leaf extract to mice induced-alloxan considerably lessened brain damage. Histopathological study revealed less inflammation and fewer indications of brain cell degeneration. The kersen leaf extract also demonstrated efficacy in reducing blood glucose levels, indicating that it may have anti-hyperglycemic properties. Kersen leaf extract demonstrates significant protective effects against alloxan-induced brain injury in mice. These findings support the use of kersen leaves as a potential alternative medicine for liver protection and management of hyperglycemia Keywords: Blood Glucose Levels, brain microstructure, hyperglicemia, medicinal plants Share Link | Plain Format | Corresponding Author (Listya Hanum Siswanti)

21	Herbal Medicine	ABS-14
The Self-nanoemulsifying (SNE) formula contains honey and clove oil as a wound healing therapy Meta Maulida Damayanti, (a), Yuniarti (b), Caecielia Makaginsar c) a) Pathology Anatomy Department, Faculty of Medicine, Universitas Islam Bandung, Bandung b) Anatomy Department, Faculty of Medicine Universitas Islam Bandung, Bandung Indonesia c) Department of Medical Education, Bioethics, Humanities and Islamic Insert, Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia Abstract Phytochemicals derived from plants have been studied in medical practice and proven to have many benefits. Inflammatory skin diseases require safe, fast-acting, and good healing results for treatment materials. Pharmaceutical research is focused on designing new drug delivery systems to increase the bioavailability of drugs that are poorly soluble in water. The self-nanoemulsifying (SNE) drug delivery system, one of the lipid-based dosage forms, has proven promising in increasing solubility, permeability and avoiding first-pass metabolism by increasing lymphatic transport. The purpose of this study is to analyze the effectiveness of the use of Self-nanoemulsifying (SNE) containing honey and cloves (Syzygium aromaticum) as a skin wound healing therapy. This study is a form of effort to reveal scientific phenomena in medical science, so that it can be a reference and alternative material for the use of natural ingredients in inflammatory skin diseases. This research method uses a true-experiment design on 27 rats (Rattus novergicus) that were induced with wounds on the back area using a sharp knife and then divided into three treatment groups. The manufacture of SNE combination of honey and clove oil was carried out, and characterization and testing of candidate drug materials were carried out, then continued with application to experimental animals for four weeks with different observation times, namely days 7, 14, and 28. Histopathological examination of skin tissue was carried out to see the effectiveness of wound healing. The significance criteria used were p values <0.05. Data analysis used two-way Anova and comparison of each group using the chi-square test. The data obtained were analyzed using SPPS 26.0 software. Keywords: Histopathology- skin inflammation- wound healing- self-nanoemusifying Share Link | Plain Format | Corresponding Author (Meta Damayanti)

22	Herbal Medicine	ABS-15
Antioxidant Activity of Ethanol Extracts from Red Ginger (Zingiber officinale R) and Lemongrass (Cymbopogon citratus) Muhamad Nurel Galbi 1, Tryando Bhatara 2, Noormartany 3, Lelly Yuniarti 4 Faculty of Medicine, Universitas Islam Bandung, Indonesia Department of Medical Biology, Faculty of Medicine, Universitas Islam Bandung, Indonesia Department of Clinical Pathology, Faculty of Medicine, Universitas Islam Bandung, Indonesia Department of Biochemistry, Nutrition, and Biomolecular, Faculty of Medicine, Universitas Islam Bandung, Indonesia Abstract Red ginger (Zingiber officinale R) and lemongrass (Cymbopogon citratus) are widely utilized as beverages, culinary spices, and traditional medicinal agents due to their distinctive flavor and aroma profiles. Both plants contain a range of bioactive compounds, including volatile constituents such as sesquiterpenes and monoterpenoids, as well as non-volatile compounds including gingerol, shogaol, zingerone, and paradol. These compounds are recognized for their potential health benefits, particularly their antioxidant and anti-inflammatory properties. The objective of the present study was to evaluate the antioxidant activity of ethanol extracts derived from red ginger and lemongrass. The extracts were prepared using a maceration method with 95% ethanol. The antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, which measures the capacity to scavenge free radicals. Absorbance readings were obtained via spectrophotometry, employing ascorbic acid as a standard reference. The findings revealed that the red ginger extract exhibited an antioxidant capacity of 46.28 ppm, while the lemongrass extract demonstrated a capacity of 154.07 ppm. The results indicate that the ethanol extract of red ginger possesses very strong antioxidant properties, in contrast to lemongrass, which exhibited comparatively weaker antioxidant activity. These results underscore the potential of red ginger as a significant source of natural antioxidants, warranting further investigation into its health-promoting properties. Keywords: antioxidant, DPPH, red ginger, lemongrass Share Link | Plain Format | Corresponding Author (lelly yuniarti)

23	Herbal Medicine	ABS-16
Comparing Antibacterial Effectiveness of the Emulgel Formulated with Arabica Coffee Java Preanger and Doxycycline Against Cutibacterium Acnes Mia Yasmina Andarini, Mieke Hemiawati Satari, Rd. Anita Indriyanti, Pramudya Adithya, Tiara Natasya Universitas Islam Bandung Abstract Abstract. Cutibacterium acnes (C. acnes) was considered to be the most important causative agent of acne vulgaris (AV) pathogenesis. Oral antibiotics are commonly used as the first line therapy for moderate-severe AV. However, the improper use of antibiotics can contribute to the increased of resistance. With reports of increased resistance to C. acnes bacteria, the management of AV has encountered many problems. The emulgel of Java Preanger Arabica coffee contains a chlorogenic acid molecule with antibacterial properties against C. acnes. The purpose of this study was to determine the antibacterial effect of the emulgel of Java Preanger Arabica coffee and Doxycycline on C. acnes. This study used an in vitro experimental design using C. acnes bacterial isolates. There were four research groups, each consisting of two concentrations of Java Preanger Arabica coffee: 75% and 100%, along with positive control group treated with Doxycycline. These groups were incubated for 24 hours at 37oC, and the inhibition zone diameter was measured in millimeters. The median results indicated that the inhibition zone at a concentration of 100% was 21.72 mm, while at the concentration of 75%, it measured 22.2 mm. In the case of Doxycycline, the median inhibition zone was formed 29.08 mm. The effectiveness of emulgel containing Java Preanger Arabica and Doxycycline at concentrations of 100% and 75% yielded a p value-of 0.001, indicating a significant difference between variables. In conclusion, there is a significant difference in antibacterial effectiveness when comparing emulgel of Java Preanger Arabica coffee and Doxycycline against C. acne. Keywords: Antibacterial, Arabica coffee, C. acnes, Doxycycline Share Link | Plain Format | Corresponding Author (mia yasmina andarini)

24	Herbal Medicine	ABS-21
Comparative Tyrosinase Inhibitory Activity of Red Ginger (Zingiber officinale R) and Lemongrass (Cymbopogon citratus) Ethanol Extracts Lelly Yuniarti,1 Maya Tejasari 2,Miranti Kania Dewi3, Erni Maryam4 Department of Biochemistry, Nutrition, and Biomolecular, Faculty of Medicine, Universitas Islam Bandung, Indonesia Department of Histology, Faculty of Medicine, Universitas Islam Bandung, Indonesia Department of Pharmacology, Faculty of Medicine, Universitas Islam Bandung, Indonesia Master^s Program in Antiaging and Medical AestheticsMedical Faculty of Universitas Jendral Ahmad Yani Abstract Abstract Melasma is characterized by dysregulated melanin production, resulting in symmetrical hyperpigmented macules with irregular borders. These manifestations commonly appear as dark brown patches on the forehead, cheeks, upper lip, and chin. Pigmentation disorders are reported to be among the top five most common skin conditions, with melasma being the most prevalent. Current treatments for melasma primarily focus on inhibiting melanin synthesis, specifically by targeting the enzyme tyrosinase and melanocytes. This study aimed to evaluate the inhibitory effects of ethanol extracts of red ginger (Zingiber officinale) and lemongrass (Cymbopogon citratus) on tyrosinase activity. The tyrosinase inhibition assay was conducted to assess the ability of various substances to inhibit melanin synthesis by targeting the activity of the tyrosinase enzyme. The results of this assay are typically expressed as IC50 (the concentration required to inhibit 50% of enzyme activity) or in kojic acid equivalents, providing a comparative measure of inhibitory potency relative to kojic acid, a widely recognized and established tyrosinase inhibitor. The results showed that the IC50 for the lemongrass ethanol extract was 281.18 ppm, while the IC50 for the red ginger ethanol extract was 181.90 ppm. In conclusion, both the ethanol extracts of red ginger and lemongrass demonstrated tyrosinase inhibitory activity and have the potential to be developed as stable anti-melasma formulations. Keywords: lemosgrass, melanin, melasma, red ginger, tyrosinase inhibitor Share Link | Plain Format | Corresponding Author (lelly yuniarti)

25	Herbal Medicine	ABS-32
Antibacterial Potential of Ethanol Extract from Butterfly Pea Flower (Clitoria ternatea L.) Against Cutibacterium acnes in the Treatment of Acne Vulgaris Joko Susanto,Gading Mangku Anugerah Universitas Islam Bandung Abstract Acne vulgaris is a long-term inflammatory skin condition affecting the pilosebaceous units, primarily caused by the commensal bacterium Cutibacterium acnes. With a prevalence of approximately 9.4% in the global population, acne vulgaris ranks as the eighth most common skin disorder. Systemic oral antibiotic therapy is a crucial option for managing acne that is unresponsive to topical treatments. However, resistance of C. acnes to antibiotics poses a global challenge in the treatment of acne vulgaris. This study aims to analyze the antibacterial activity of ethanol extract from butterfly pea flower (Clitoria ternatea L.) against C. acnes. Antibacterial testing was conducted by measuring the inhibition zones on agar medium at concentrations of 100%, 80%, 60%, 40%, and 20%, alongside determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) through dilution assays. Results indicate that the ethanol extract of butterfly pea flower produced inhibition zones at concentrations of 80%, 60%, 40%, and 20%, with respective sizes of 14,45mm, 12,85mm, 11,82mm and 10,42mm. The dilution assays revealed that the MIC of the extract was 0.318%, while the MBC was found to be 1.25%. Conclusion: The ethanol extract of Clitoria ternatea L. exhibits moderate antibacterial activity against C. acnes, suggesting its potential for development as an acne treatment formulation. Further research is necessary to elucidate the mechanisms of action and practical applications of this extract in the therapy of acne vulgaris. Keywords: Herbal medicine Share Link | Plain Format | Corresponding Author (Joko Susanto)

26	Herbal Medicine	ABS-33
Antihypercholesterolemic Activity of Ethanol Extract from Red Ginger (*Zingiber officinale var. rubrum Rhizoma) in Male Mice Model of Hypercholesterolemia Gading Mangku Anugerah, Joko Susanto Undergraduate Program, Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia Abstract *Abstract* Uncontrolled elevation of plasma cholesterol levels is a significant risk factor for various cardiovascular diseases, including hypertension, stroke, ischemic heart disease, and atherosclerosis. Furthermore, it increases the likelihood of chronic kidney injury, acute pancreatitis, and retinal lipemia. Statins are the conventional pharmacological agents employed in the management of hypercholesterolemia- however, their therapeutic application is frequently accompanied by adverse effects, including myopathy, liver dysfunction, renal insufficiency, diabetes, and ocular complications. This study aims to rigorously evaluate the antihypercholesterolemic efficacy of ethanol extract derived from red ginger (Zingiber officinale var. rubrum) in a hypercholesterolemic mouse model. The investigation was designed as an in vivo experimental study utilizing a randomized allocation methodology involving 30 male Swiss Webster mice (Mus Musculus). Hypercholesterolemia was induced in the subjects through a high-fat dietary regimen. The administration of the ginger extract was conducted over a 14-day period, during which plasma cholesterol levels were quantified employing enzymatic assays and analyzed using spectrophotometric techniques. The data obtained were subjected to statistical analysis using ANOVA, adhering to a 95% confidence interval. The results indicated that hypercholesterolemic mice receiving the ethanol extract of red ginger exhibited a statistically significant reduction in cholesterol levels compared to control groups that did not receive the extract. *Conclusion*: The findings of this study suggest that ethanol extract from red ginger possesses substantial antihypercholesterolemic properties in a hypercholesterolemic mouse model, thereby underscoring its potential as an alternative therapeutic intervention for the management of hypercholesterolemia. Keywords: Share Link | Plain Format | Corresponding Author (Gading Mangku Anugerah)

27	Herbal Medicine	ABS-38
The Effect of Ethyl Acetate Extract of Bay Leaf (Syzygium Polyanthum Wight) on Leukocyte and C-Reactive Protein (CRP) Levels in Male Hyperuricemic Rat Models Miranti Kania Dewi, dr., MSi., Dr. Santun Bhekti Rahimah, dr., MKes., Wahidah, Salfa Azizah Giyatsilah, Finarsih Medical Faculty of Universitas Islam Bandung Abstract Hyperuricemia characterized by elevated levels of uric acid in the blood, which trigger inflammatory processes within the body. This inflammation may lead to increased levels of leukocytes and C-reactive protein in the blood. Bay Leaf (Syzygium Polyanthum Wight) possesses properties that reduce uric acid levels in serum, indirectly inhibiting inflammation. This study aims to investigate the effects of ethyl acetate extract of Bay Leaf on leukocyte and CRP levels using experimental study with a completely randomized design. The research subjects were male rats meeting the inclusion criteria: male, age of 2-3 months, weight of 200-300 grams, healthy, and no prior use in research. A total of 24 rats were used, divided into five groups: a negative control, a positive control, and three treatment groups induced with a high-purine diet and potassium oxonate injections, followed by the administration of ethyl acetate Bay Leaf extract at varying doses (210 mg/kgBW/day, 420 mg/kgBW/day, and 840 mg/kgBW/day). Leukocyte and CRP levels were measured at the end of the treatment period. Results of this study show that all groups exhibited CRP levels below 1.0 mg/dL, indicating no significant systemic inflammation. Leukocyte counts were elevated in the negative control group, reflecting an uncontrolled inflammatory response. Treatment with ethyl acetate extract of bay leaves demonstrated a dose-dependent reduction in leukocyte counts, with the highest dose showing the most significant anti-inflammatory effect. The ethyl acetate extract of bay leaves effectively maintained low CRP levels and reduced leukocyte counts, highlighting its potential as an anti-inflammatory agent for hyperuricemia. Keywords: C-reactive protein- bay leaf- ethyl acetate extract- hyperuricemia- leukocytes Share Link | Plain Format | Corresponding Author (Miranti Kania Dewi)

28	Herbal Medicine	ABS-45
Screening and Characterization of Endophytic Molds in Increasing the Content of Isoeugenol Compounds in Citronella Plants (Cymbopogon Winterianus) Dwi Ningsih Susilowati 1), Nani Radiastuti2), Firda Indraswati,2) 1Indonesian Center for Agricultural Biotechnology and Genetic Resouces Research and Development, West Java, Indonesia. 2Department of Biology Faculty of Science and Technology, UIN Syarif Hidayatullah Jakarta. Jl. Ir. H. Djuanda no 90, Ciputat, Banten. Indonesia. Abstract Citronella plants can produce essential oils that contain active compounds such as citronellal, citronellol, geraniol, and isoeugenol. The isoeugenol production has a higher selling price than the main active compound. This research aims to obtain endophytic fungi isolates from citronella plants, which can potentially increase the content of isoeugenol compounds through endophytic fungi inoculation treatment. Analysis of isoeugenol compound content of citronella plant using GC-MS isolates with content above control was followed by molecular investigation including BLAST, phylogenetic, and characterization of endophytic fungi. The results of molecular identification using ITS1 and ITS4 primers on 5 endophytic fungi increased the content of isoeugenol compounds in control plants. Endophytic fungi and their increasing percentage were Clonostachys Rosea, Curvularia lunata, Cladosporium cladosporioides, Gaeumannomyces graminis, and Aspergillus flavus at 8.25%, 7.28%, 7.52%, 20.87%, and 0.24%, respectively. The highest increase in isoeugenol compounds was found in the endophytic G. graminis by 20.87%. Endophytic fungi increased the production of isoeugenol compounds in citronella plants. Keywords: Cymbopogon winterianus- Phenylalanine ammonia-lyase- Gaeumannomyes graminis- GCMS- isoeugenol Share Link | Plain Format | Corresponding Author (Nani Radiastuti)

29	Herbal Medicine	ABS-51
Aspergillus flavus ISOLATED FROM Bauhinia Purpurea Linn. AND THE CYTOTOXIC ACTIVITY AGAINST BREAST CANCER CELL LINES MICHIGAN CELL FOUNDATION-7 (MCF-7) Teja Koswara 1, Sutrisno 2, Jasmansyah 3, Sari Purbaya 3, Anceu Murniati 3, Hernandi Sujono 3, Atik Nuraisyah 3 1. Departement of, Anatomical Pathology, Faculty of Medicine Universitas Jenderal Achmad Yani, Cimahi, Indonesia. 2. Departement of bioethics, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia. 3. Departement of chemistry, Faculty of Science and Informatics, Universitas Jenderal Achmad Yani, Cimahi, Indonesia Abstract Breast cancer ranks first among the most common types of cancer in Indonesia and is one of the first contributors to cancer deaths. Bauhinia purpurea Linn. is a family of Fabaceae which is known to be rich in bioactive compounds and pharmacological effects such as antioxidant, hepatoprotective, hypoglycemic and antiproliferative. The anticancer activity of B. purpurea L. against MCF-7 breast cancer cells with MTT assay method has also been reported. Endophytic fungi are fungi that live in colonies in healthy plant tissue. Endophytic fungi also produce the same bioactive compounds as their host plants. The aim of this research was to determine the cytotoxic activity against MCF-7 breast cancer cells from the endophytic fungus of the plant B. purpurea L. The initial stage was cultivating the endophytic fungus from the leaves of B. purpurea L. and identifying it molecularly, then fermenting the endophytic fungus and obtaining an extract. media. The media extract was carried out using a liquid-liquid extraction method using ethyl acetate, then a cytotoxicity test was carried out on MCF-7 breast cancer cells from the concentrated ethyl acetate extract. Aspergillus flavus is an endophytic fungus that was isolated from the leaves of B. purpurea L. and an IC50 value of 1810.88 &#956-g/mL was obtained, indicating that the bioactivity of the endophytic fungus of B. Purpurea L. plants is included in the non-cytotoxic category. Keywords: Key words : B. purpurea L, A. flavus, cytotoxic activity, endophytic, MCF-7 Share Link | Plain Format | Corresponding Author (Teja Koswara)

30	Herbal Medicine	ABS-76
Nanoparticle Gel of Crustaceans as Scaffold for Periodontal Tissue Regeneration Yuniarti,Meta Maulida Damayanti,Ratih Aryani Faculty of Medicine, Universitas Islam Bandung Abstract Periodontal tissue regeneration therapy is currently being developed due to the high number of periodontal diseases. One of the important components of bone tissue engineering is the development of innovative bone substitute biomaterials for bone transplantation to correct orthopedic deformities. Chitosan, a natural biopolymer produced mainly from crustaceans, has attracted much interest due to its beneficial properties, including biocompatibility, biodegradability, and osteoconductive. This study aims to analyze the effectiveness of crustacean nanoparticle gel as a scaffold for periodontal tissue regeneration. The preparation of crustacean nanoparticles was carried out using ionic gelation method to test Particle Size Analyzer (PSA), Zeta Potential Analyzer (ZPA), and Transmission Electron Microscope (TEM) tests. The physicochemical parameters of chitosan gel were assessed organoleptically, homogeneity, pH, and viscometer. The results of the PSA, ZPA, and TEM tests of crustacean extract nanoparticle showed that the preparation successfully met the stable requirements as nanoparticles with a size of less than 1000 nm and a zeta potential of more than -30mV. Based on the results of the characterization test, the crustacean nanoparticle extract gel had a homogeneous gel with the highest spreadability in the 10% crustacean nanoparticle gel, and the pH of the gel had met the requirements for application to the oral mucosa. The right pH for application on the oral mucosa is in the range of pH 4-6.5. The results of crustacean gel characterization showed that chitosan was synthesized effectively with optimal physicochemical properties. 10% crustacean nanoparticles are suitable injectable substances for periodontal regeneration. Keywords: periodontal tissue, regeneration, crustaceans Share Link | Plain Format | Corresponding Author (yuniarti yuniarti)

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