Soyghurt Potentially Controls the Level of sFlt1 and PLGF in Preeclampsia Maternal Serum-Induced Placental Trophoblast Cells in Vitro Astrid Feinisa Khairani1, Uci Ary Lantika2, Julia Ramadhanti1, Muhammad Hasan Bashari1,3, Widad Aghnia Shalannandia4, Tenny Putri Wikayani5, Achadiyani Achadiyani1, Mulyanusa Amarullah Ritonga6
1Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Jatinangor, West Java, Indonesia-
2Department of Biomedical Sciences, Faculty of Medicine, Universitas Islam Bandung, Bandung, West Java, Indonesia-
3Research Centre of Oncology and Stem Cell, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia-
4Graduate School of Master Program in Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Indonesia,
5Culture and Cytogenetic Laboratory, Universitas Padjadjaran, Bandung, West Java, Indonesia-
6Department of Obstetrics and Gynecology, Hasan Sadikin General Hospital, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia
Abstract
Background: Soyghurt is a symbiotic drink that consists of probiotics as the fermentation agent, which could increase the beneficial effect of soybeans. Preeclampsia is one of the contributors to mortality in pregnant women and their children worldwide. Escalation of inflammation, oxidative stress, and endothelial dysfunction are the aspects that promote an imbalance condition of angiogenic substance (sFLT-1) and antiangiogenic (PLGF) in preeclampsia. One of the prospective ways to prevent preeclampsia is by utilizing functional food such as soyghurt. This study aims to evaluate the effect of soyghurt supernatant on other preeclampsia markers (sFLT-1 and PLGF) levels in the primary placenta culture, which had been induced by preeclampsia serum.
Methods: The trophoblast primary culture was induced by preeclampsia serum (10%). The Soyghurt treatment was performed with 2.5%, 5%, and 7.5% Soyghurt supernatant concentrations in culture media. The expression of preeclampsia markers, sFLT-1, and PLGF were evaluated using ELISA.
Results: Expression sFLT-1 on preeclampsia-induced cell culture treated with Soyghurt was significantly lowered compared to the untreated group (p<0.01). However, no significant difference was observed in the PLGF levels of all groups induced by preeclampsia serum (p>0.05).
Conclusion: This study demonstrates the potential impact of Soyghurt in balancing preeclampsia marker expression by inhibiting the expression of sFLT-1 in preeclampsia serum-induced trophoblast cells.
