The Effect of Ethyl Acetate Extract of Bay Leaf (Syzygium Polyanthum Wight) on Leukocyte and C-Reactive Protein (CRP) Levels in Male Hyperuricemic Rat Models Miranti Kania Dewi, dr., MSi., Dr. Santun Bhekti Rahimah, dr., MKes., Wahidah, Salfa Azizah Giyatsilah, Finarsih
Medical Faculty of Universitas Islam Bandung
Abstract
Hyperuricemia characterized by elevated levels of uric acid in the blood, which trigger inflammatory processes within the body. This inflammation may lead to increased levels of leukocytes and C-reactive protein in the blood. Bay Leaf (Syzygium Polyanthum Wight) possesses properties that reduce uric acid levels in serum, indirectly inhibiting inflammation. This study aims to investigate the effects of ethyl acetate extract of Bay Leaf on leukocyte and CRP levels using experimental study with a completely randomized design. The research subjects were male rats meeting the inclusion criteria: male, age of 2-3 months, weight of 200-300 grams, healthy, and no prior use in research. A total of 24 rats were used, divided into five groups: a negative control, a positive control, and three treatment groups induced with a high-purine diet and potassium oxonate injections, followed by the administration of ethyl acetate Bay Leaf extract at varying doses (210 mg/kgBW/day, 420 mg/kgBW/day, and 840 mg/kgBW/day). Leukocyte and CRP levels were measured at the end of the treatment period. Results of this study show that all groups exhibited CRP levels below 1.0 mg/dL, indicating no significant systemic inflammation. Leukocyte counts were elevated in the negative control group, reflecting an uncontrolled inflammatory response. Treatment with ethyl acetate extract of bay leaves demonstrated a dose-dependent reduction in leukocyte counts, with the highest dose showing the most significant anti-inflammatory effect. The ethyl acetate extract of bay leaves effectively maintained low CRP levels and reduced leukocyte counts, highlighting its potential as an anti-inflammatory agent for hyperuricemia.
Keywords: C-reactive protein- bay leaf- ethyl acetate extract- hyperuricemia- leukocytes
