Acute and sub-chronic oral toxicity study of purple sweet potato (Ipomoea batatas [L.] Lam) yogurt in mice (Mus musculus)
Astrid Feinisa Khairani (a), Yunisa Pamela (a), Nandina Oktavia (a*), Achadiyani Achadiyani (a), M. Yusuf Adipraja (b), Prita Yasri Zhafira (b), Widad Aghnia Shalannandia (a), Nur Atik

a. Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, West Java, Indonesia- b. Undergraduate Program, Faculty of Medicine, Universitas Padjadjaran, West Java, Indonesia.

Abstract

The purple sweet potato yogurt (PSPY) product was developed to combine the benefits of probiotic activities in yogurt and the bioactive effects of anthocyanin in purple sweet potato. Aspect of food safety important to be evaluated in preventing any potentially harmful side effects of food product. This study aims to investigate acute and sub-chronic oral toxicity of PSPY in mice. Methods used to evaluate acute oral toxicity was by a 14-days observation for any clinical sign of toxicity on fifteen female balb/c mice following a single dosage of PSPY (nil, 2 or 5 g/kg body weight), whilst for the sub-chronic oral toxicity was conducted to four groups of mice with the dose of 0, 12, 20, and 40 g/kg body weight for 28 days, and another group of mice with 40 g/kg body weight for 14 days longer to observe any delayed toxicity effect. Daily observation was made on body weight and clinical signs of toxicity. Study about the liver and kidney macroscopy, microscopy and relative organ weight, liver enzyme, and haematology profile analyses were done at the end of the study. Results found there were no signs of acute toxicity observed and no abnormality in body weight, relative organ weight, and gross organ examination. In the sub-chronic toxicity found no clinical signs of toxicity, no significant differences in body weight, relative liver weight, liver enzymes, haematology profile, or abnormality in gross and histological examination of the liver. Conclusion of study found that oral administration of PSPY in mice did not result in acute toxicity, while the dosage up to 40 g/kg body weight did not lead to sub-chronic toxicity.

Keywords: acute oral toxicity, anthocyanin, purple sweet potato, sub-chronic oral toxicity, yogurt

Topic: Genetic and Molecular Biology