Does CCL4 Effective to Induce Acute Kidney Injury in Wistar Rats? Tita Barriah Siddiq1, a), Meta Maulida Damayanti2, b), Ismet Muchtar Nur2, c), Ratu Adeline3,
1Department of Medical Education, Bioethics and Humanity, Faculty of Medicine, Universitas Islam Bandung, Tamansari Bandung, West Java, Indonesia
2Department of Pathology Anatomy, Faculty of Medicine, Universitas Islam Bandung, Tamansari Bandung, West Java, Indonesia
3Universitas Islam Bandung, Tamansari Bandung, West Java, Indonesia
Abstract
Acute kidney injury (AKI) is described as the sudden loss of renal excretory function. AKI is one of a group of diseases characterized as acute kidney diseases and disorders, in which progressive decline in kidney function or persistent kidney dysfunction is associated with irreversible loss of kidney cells and nephrons. Nephrotoxicity induced by chemicals and drugs is the main cause of acute kidney injury. Carbon tetrachloride (CCl4) is the most potent toxin, and is widely used in scientific studies to create experimental models that simulate oxidative stress in various pathophysiological scenarios. The purpose of this study was to determine whether CCL4 can effectively cause kidney injury in rats. This study was a true experimental study using eighteen adults male Wistar rats weighing between 200 and 300 g, which were induced by CCL4 for kidney injury. Kidney function tests and histopathological image analysis were performed. The results showed that there was an increase in Blood Urea Nitrogen (BUN) and serum creatinine levels in the treatment group compared to the control group with p <0.05, and histopathological found kidney damage in the form of atrophic tubules with necrotic lining cells. It can be concluded that CCL4 is a fairly strong nephrotoxic material for kidney injury that causes oxidative stress by reducing the activity of antioxidant enzymes, the release of inflammatory cytokines, and apoptosis in the treatment of nephrotoxicity due to drugs or chemicals.
