Quantitative Measurement of Langerhans Islet Impairment of Diabetic Rat Model With Cigarete Exposure
Yuktiana Kharisma,1 Yuke Andrianne,2 Abdul Hadi Hassan,1 Ratih Syifa H3

1 Anatomic Pathology Department, Medical Faculty oF Universitas Islam Bandung, Indonesia
2 Pharmacology Department, Medical Faculty oF Universitas Islam Bandung, Indonesia
3 Undergraduate Program, Medical Faculty oF Universitas Islam Bandung, Indonesia

Abstract

Diabetes mellitus and cigarette smoke exposure are dominant health issue around the world. These conditions affect the organs, especially pancreas. This study aims to evaluate the impairment of Langerhans islet in diabetic rats model exposed to cigarette smoke. This study evaluates the density, area, and cell count within Langerhans islet diabetic rats exposed to cigarette smoke.
A total of 18 rats were divided into 3 groups (n=6 per group). Group 1 was healthy rats (control), group 2 was diabetic rats exposed to cigarette smoke, and group 3 was treated with glibenclamide and simvastatin. Pancreatic tissue was collected and stained using Hematoxylin-Eosin (HE) with 100x, 400x magnification. Data normality was assessed using the Shapiro-Wilk test. One-Way ANOVA was used to compare the density and cell count, while the Kruskal-Wallis test was applied to Langerhans islet area. Significant differences in islet density were identified (p< 0.01). However, no significant differences were observed in area and cell count across groups (p=0.33) and (p=0.22). Bonferroni post hoc test revealed significant differences between groups 1 and 2 (p=0.002), groups 1 and 3 (p=0.003), but no significant difference between groups 2 and 3 (p=1.000). Diabetic with cigarrete exposure impact significantly the quantitative measurement of Langerhans islet especially the density of Langerhans islet. Diabetic and cigarrete induces exacerbating cellular damage by increased reactive oxygen species production, contributing to impairement of pancreatic cell. Simvastatin and glibenclamide appear to mitigate these adverse effects, suggesting their potential utility in managing diabetes complicated by smoking.

Keywords: Diabetic, Langerhans Cell, Pancreas, Smoking

Topic: Degenerative Disease