Nanoparticle Gel of Crustaceans as Scaffold for Periodontal Tissue Regeneration Yuniarti,Meta Maulida Damayanti,Ratih Aryani
Faculty of Medicine, Universitas Islam Bandung
Abstract
Periodontal tissue regeneration therapy is currently being developed due to the high number of periodontal diseases. One of the important components of bone tissue engineering is the development of innovative bone substitute biomaterials for bone transplantation to correct orthopedic deformities. Chitosan, a natural biopolymer produced mainly from crustaceans, has attracted much interest due to its beneficial properties, including biocompatibility, biodegradability, and osteoconductive. This study aims to analyze the effectiveness of crustacean nanoparticle gel as a scaffold for periodontal tissue regeneration. The preparation of crustacean nanoparticles was carried out using ionic gelation method to test Particle Size Analyzer (PSA), Zeta Potential Analyzer (ZPA), and Transmission Electron Microscope (TEM) tests. The physicochemical parameters of chitosan gel were assessed organoleptically, homogeneity, pH, and viscometer. The results of the PSA, ZPA, and TEM tests of crustacean extract nanoparticle showed that the preparation successfully met the stable requirements as nanoparticles with a size of less than 1000 nm and a zeta potential of more than -30mV. Based on the results of the characterization test, the crustacean nanoparticle extract gel had a homogeneous gel with the highest spreadability in the 10% crustacean nanoparticle gel, and the pH of the gel had met the requirements for application to the oral mucosa. The right pH for application on the oral mucosa is in the range of pH 4-6.5. The results of crustacean gel characterization showed that chitosan was synthesized effectively with optimal physicochemical properties. 10% crustacean nanoparticles are suitable injectable substances for periodontal regeneration.
