Aspergillus flavus ISOLATED FROM Bauhinia Purpurea Linn. AND THE CYTOTOXIC ACTIVITY AGAINST BREAST CANCER CELL LINES MICHIGAN CELL FOUNDATION-7 (MCF-7) Teja Koswara 1, Sutrisno 2, Jasmansyah 3, Sari Purbaya 3, Anceu Murniati 3, Hernandi Sujono 3, Atik Nuraisyah 3
1. Departement of, Anatomical Pathology, Faculty of Medicine Universitas Jenderal Achmad Yani, Cimahi, Indonesia.
2. Departement of bioethics, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia.
3. Departement of chemistry, Faculty of Science and Informatics, Universitas Jenderal Achmad Yani, Cimahi, Indonesia
Abstract
Breast cancer ranks first among the most common types of cancer in Indonesia and is one of the first contributors to cancer deaths. Bauhinia purpurea Linn. is a family of Fabaceae which is known to be rich in bioactive compounds and pharmacological effects such as antioxidant, hepatoprotective, hypoglycemic and antiproliferative. The anticancer activity of B. purpurea L. against MCF-7 breast cancer cells with MTT assay method has also been reported. Endophytic fungi are fungi that live in colonies in healthy plant tissue. Endophytic fungi also produce the same bioactive compounds as their host plants. The aim of this research was to determine the cytotoxic activity against MCF-7 breast cancer cells from the endophytic fungus of the plant B. purpurea L. The initial stage was cultivating the endophytic fungus from the leaves of B. purpurea L. and identifying it molecularly, then fermenting the endophytic fungus and obtaining an extract. media. The media extract was carried out using a liquid-liquid extraction method using ethyl acetate, then a cytotoxicity test was carried out on MCF-7 breast cancer cells from the concentrated ethyl acetate extract. Aspergillus flavus is an endophytic fungus that was isolated from the leaves of B. purpurea L. and an IC50 value of 1810.88 μ-g/mL was obtained, indicating that the bioactivity of the endophytic fungus of B. Purpurea L. plants is included in the non-cytotoxic category.
Keywords: Key words : B. purpurea L, A. flavus, cytotoxic activity, endophytic, MCF-7
