Impact of post heat treatment on the Crystallinity of hydroxyapatite Synthesized from Limestone and the adhesion of various Gram Positive and Gram Negative Bacteria Evi Ulina Margareta Situmorang1,2, Heri Sutanto3, Parsaoran Siahaan4, Nyoman Jujur5, Giri Wahyu Alam5, Daniel edbert6, Ferry Budhi Susetyo7, Nendar Herdianto5, Widodo Widjaja Basuki8, Yustinus Dimas Gianni Prabowo8
1 Doctoral Degree Mathematics and Science, Mathematics and Science Faculty, Universitas Diponegoro, 50275, Indonesia
2Department of Physiology School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, 14440, Indonesia
3Department of Physics, Universitas Diponegoro, 50275, Indonesia
4Department of Chemistry, Universitas Diponegoro, 50275, Indonesia
5Research Center for Advanced Materials-National Research and Innovation Agency, 15314, Indonesia
6Department of Microbiology, Atma Jaya Catholic University of Indonesia, 14440, Indonesia
7Department of Mechanical Engineering, Universitas Negeri Jakarta, 13220, Indonesia
8Department of Mechanical Engineering, Atma Jaya Catholic University of Indonesia, 14440, Indonesia
Abstract
The study investigates the impact of post-heat treatment on the structural properties of hydroxyapatite (HA) and its effect on bacterial adhesion, specifically against Escherichia coli and Staphylococcus aureus. Hydroxyapatite was synthesized via a hydrothermal method using natural limestone as the calcium source. The samples were calcined at 700C, 900C, and 1100C for 1 hour. Comprehensive characterization was conducted using X-ray Diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM). The results showed that near-stoichiometric and highly crystalline HA was obtained at 900-C. Higher calcination temperatures resulted in increased grain size and significant surface morphological changes. These surface modifications altered the interfacial characteristics of HA, which significantly reduce affects the adhesion of both E. coli and S. aureus. The study demonstrates that post-heat treatment not only improves the crystallinity of HA but also performance of hydroxyapatite for biomedical applications.
Keywords: Hydroxyapetite, heat treatment, bacteria adhesion, material characteritationPlease Just Try to Submit This Sample Abstract
