Proliferation 10% Nano Chitosan and Platelet Rich Plasma from Preosteoblast Cell with Ki67 as in Vitro Surugate Marker Dewa Made Wedagama (a*), G.A.A Hartini (a)
a) Departement Conservative, Faculty of Dentistry, Universitas Mahasaraswati Denpasar
*wedagama_fkg[at]unmas.ac.id
Abstract
This study investigated the effect of nanochitosan mix with platelet rich plasma (PRP) 0% and 10% to proliferation rate of preosteoblast cell with incubation time of five and seven. The culture media of pre-osteoblast cell MC3T3-E1 (ATCC murine cell line) used alpha-MEM, 2mm L-glutamine, 1mm sodium pyruvate, 10% FBS and 10% penstrep in 25 cm2 flask bottle and incubated in an incubator with 5% CO2 at a temperature of 37oC until the cell was confluent 70-80%. And cells were ready for planting in well-24 to give treatments. The treatment was divided into two groups, nanochitosan+PRP and hydroxyapatite+PRP. The proliferation of preosteoblast cell saw with immunocytochemical staining and proliferation of cells were investigated with confocal laser scanning microscope (CLSM). Comparison test used independent sample t-test and one-way ANOVA test. The experiment results showed that nanochitosan+PRP can accelerate proliferation than Hydroxyapatite+PRP of 0% and 10% concentrations. The independent sample t-test showed there were a significant difference (p=0,010) from proliferation rate mean (0%) between treatment group nanochitosan+PRP (1076,3+176,4) and treatment group hydroxyapatite+PRP (659,5+272,7) on five days incubation time, and proliferation rate mean (10%) between treatment group of nanochitosan+PRP (710,3+109,7) and hydroxyapatite+PRP (581,8+76,4) on seven days incubation time. Based on proliferation mean rate (0%) and (10%), the treatment group of nanochitosan+PRP with five and seven days incubation have higher mean than 0% and 10% on treatment group nano chitosan and PRP and can accelerate bone healing with incubation time of five and seven days compared to treatment group of hydroxyapatite+PRP
