In Vitro Antibacterial Activity of Combined Extract of Hylocereus polyrhizus and Spatholobus littoralis Hassk. Devina Alifia Andrini(1), Yessy Ariesanti(2*), Wiwiek Poedjiastoeti(2), Anggraeny Putri Sekar Palupi(2), Muhammad Ihsan Rizal(3)
1 Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia
2 Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia.
3 Department of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia.
Abstract
Background: Tooth extraction, a common dental procedure, can lead to complications including bacterial infection. Previous researches have proved that separate extracts from Hylocereus polyrhizus peel and Spatholobus littoralis Hassk. stem have antibacterial properties. Objective: This study aims to investigate the potential antibacterial activity of combining Hylocereus polyrhizus peel and Spatholobus littoralis stem against Staphylococcus aureus and Escherichia coli. Method: The study used the well diffusion and plate count methods to test the combined extracts (1:1) antibacterial activity against Staphylococcus aureus and Escherichia coli. The following concentrations of the extract were prepared: 3.125%, 6.25%, 12.5%, 25%, 50%, 75%, and 100%. In this study, aquadest served as the negative control and ciprofloxacin 50 microgram/mL as the positive control. Result: The results demonstrated that extract formed inhibition zones against Staphylococcus aureus with diameters of 13.68 mm (100%), 13.1 mm (75%), 12.18 mm (50%), and 10.52 mm (25%), while for Escherichia coli it was 11.19 mm (100%), 10.34 mm (75%), 8.57 mm (50%), and 7.84 mm (25%). The minimum inhibitory concentration (MIC) of the extract against both bacteria was determined to be 6.25%, while the minimum bactericidal concentration (MBC) was 100%, indicating a bacteriostatic effect at 6.25%, 12.5%, 25%, and 50%, and a bactericidal effect at 100%. Overall, the extract showed a stronger antibacterial effect against Staphylococcus aureus compared to Escherichia coli. Conclusion: A combined extract of Hylocereus polyrhizus peel and Spatholobus littoralis stem has antibacterial activity against Staphylococcus aureus and Escherichia coli.
