Limosilactobacillus reuteri and Hyaluronic Acid Combination Effects on Macrophage and Fibroblast Proliferation Post-Tooth Extraction Desak Made Hari Wijayanti* Armelia Sari Widyarman**, Melanie Hendriaty Sadono**
*Postgraduate Student, Faculty of Dentistry, Universitas Trisakti, Indonesia
**Department of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Indonesia
Abstract
Background: Limosilactobacillus reuteri (L. reuteri) or hyaluronic acid (HA) has been proposed as supportive therapies to promote the wound healing process. Objective: The aim of this study was to examine the histopathological features of macrophages and fibroblasts during the wound healing process post-tooth extraction following treatment with a combination of HA and L. reuteri gel. Methods: Probiotic lozenges (L. reuteri DSM 17938 and ATCC PTA 5289) were made into a gel form. White male Wistar rats (Rattus norvegicus) were divided into five groups (HA, L. reuteri, probiotic, a combination of HA and L. reuteri, povidone-iodine (PVP-I), and a control group (no therapy and assessed on days 3, 7, and 14 post-left mandibular central incisor extraction. The histopathological appearance of macrophages and fibroblasts were analyzed using hematoxylin and eosin (H&E) staining. Results: The results revealed significant differences in the number of macrophages between the combination treatment group (L. reuteri and HA) compared with the other treatment groups on days 3, 7, and 14 (P < 0.05). The results also revealed a significant difference in fibroblast proliferation in the combination treatment group compared to other groups on days 3, 7, and 14 (P < 0.05), with the number of fibroblasts significantly increasing on days 3 and 7, followed by accelerated proliferation on day 14 which was marked a reduction in the number of fibroblasts. Conclusions: This study demonstrates the potential ability of L. reuteri combined with HA to promote wound healing post-tooth extraction by enhancing macrophage and fibroblast proliferation.
