Water Extract of Kersen Leaves Changes Islet of Langerhans Histopathological Appearance in Mice (Mus musculus) Induced by Alloxan Assakina, N. D (1), Nur Artri I (1), Putri Felita A (1), Syarah, S.W.K(1), Tejasari M (2)
(1) Undergraduate Program, Faculty of Medicine Universitas Islam Bandung, Bandung, Indonesia
(2) Histology Department, Faculty of Medicine Universitas Islam Bandung, Bandung, Indonesia
Abstract
Introduction: Alloxan is a diabetogenic substance that is toxic to pancreatic beta cells. When given to laboratory animals such as mice, it can cause the laboratory animals to develop diabetes. Based on previous studies, diabetes causes a decrease in the diameter of the Islets of Langerhans of the pancreas. This study aims to investigate the protective effects of water extract of kersen leaves (Muntingia calabura L.) on the histopathological changes of pancreas in mice (Mus musculus) induced by alloxan. Methods: There were 4 experimental groups- K1, K2, K3, and K4 with a sample size of 20 male Swiss Webster mice (Mus Musculus). Alloxan (160 mg/kgBW) was given as a hyperglycemic agent to all groups. Kersen leaves extract was given to groups K2, K3, K4 at 200, 400, and 800 mg/kgBW respectively for 14 days after alloxan induction. Results: The active ingredients in kersen leaves such as phenols, flavonoids, alkaloids, saponins, and tannins have biological activity as antioxidants that can increase the diameter of the Islets of Langerhans. Conclusion: There is an effect of water extract of kersen leaves (Muntingia calabura L.) on the histopathology of the pancreas of mice (Mus musculus) induced by alloxan.
Keywords: Muntingia calabura L., kersen leaves, water extract of kersen leaves, histopathology of the pancreas, alloxan, diabetes
