Water Extract of Kersen Leaves (Muntingia calabura L.) Prevent Cardiac Myocytes Necrosis in Mice (Mus musculus) Induced by Alloxan Putri Felita A (1), Nur Artri I (1),Assakina, N. D (1),Syarah S W K (1),Tejasari M (2)
(1) Undergraduate Program, Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia
(2) Department of Histology Faculty of Medicine, Universitas Islam Bandung, Bandung, Indonesia
Abstract
Introduction: Alloxan is one of the most common diabetogenic agents used in diabetes research to evaluate the antidiabetic potential of pure compounds and plant extracts. It has been successfully induced in several animal species, including rabbits, mice, rats, monkeys, and cats. Objective:This study aims to investigate the effects of aqueous extract of kersen leaves (Mutingia calabura L.) on the histopathological structure of the heart in alloxan-induced diabetic mice (Mus musculus). Methods: There were 4 experimental groups- K1, K2, K3, and K4 with a sample size of 20 male Swiss Webster mice (Mus Musculus). Alloxan (160 mg/kgBW) was given as a hyperglycemic agent to all groups except the normal group. Kersen leaves extract was given to groups K2, K3, K4 at 200, 400, and 800 mg/kgBW respectively for 14 days after alloxan induction. Results:The study found that kersen leaf extract significantly improved the number of normal cardiac muscle cells in mice, attributed to the antioxidant properties of flavonoids in the leaves. Flavonoids neutralize free radicals and reduce oxidative stress, thereby preventing cellular necrosis in cardiac myocytes. Conclusion: The water extract of kersen leaves exhibits potential as an alternative treatment to mitigate hyperglycemia-induced cardiac damage in diabetic mice. This study supports the use of kersen leaves in managing diabetes-related cardiovascular complications and aligns with the Sustainable Development Goals (SDGs) on good health and well-being, and industry, innovation, and infrastructure.
