Utilization of Tapioca Industrial Solid Waste (Onggok flour) as a Carbon Source in the Alternative Medium of Lactobacillus plantarum with Variation of Onggok Flour Concentration Anggarini, S., Yuflih, F
Departement of Agroindustrial Technology, Faculty of Agrcultural Technology, Brawijaya University
Abstract
Industrially, many microorganisms generate many beneficial products, including Lactobacillus plantarum (L. plantarum) bacteria. Nutrients in the medium are essential for bacterial growth. However, the price of commercial mediums is relatively high, therefore it is important to find an alternative substrate. This study proposed tapioca solid waste (onggok flour) as an alternative carbon sources. At the beginning, Onggok flour should be dissolved into water to obtain the filtrate. Cell culture was prepared with initial OD600 0.01. The bacterial growth was checked by turbidimetry and TPC method. Metabolite yields were identified by pH measurement and total titrated acid, then the sugar utilization was investigated through total reducing sugars, growth yield (Yx/s), and acid yield (Yp/s). The data were analyzed using One-way ANOVA, DMRT (α- <5%), and Zeleny^s Multiple Attributes. The results demonstrated that the best alternative medium was onggok flour in 15% (w/v), emphasized by increased OD600 was 1.412, increased cells was 72.4 CFU/mL, increased pH was 0.58, increased total titrated acid was 1.59 mg/mL (35%), decreased reducing sugar 4.53 mg/mL (91%) with a Yx/s of 1.32 CFU/mg and a Yp/s of 0.35. It is concluded that onggok flour filtrate can be an alternative medium for L. plantarum. To maximize onggok utilization, onggok flour can be pre-treated to complete the hydrolyzation.
Keywords: alternative medium, carbon source, L. plantarum, tapioca solid waste, variation of concentration
