Optimization of Ultrasound Assisted Extraction Conditions for Syringodium isoetifolium Using Response Surface Methodology Bambang Susilo*, Ilona Evangelina
Departement of Biosystem Engineering, Faculty of Agricultural Technology, Universitas Brawijaya, Veteran Street, Malang 65145, Indonesia
*susilo[at]ub.ac.id
Abstract
Syringodium isoetifolium is one of seagrass types that can be found on the coast of Indonesia. This species has potential as an antioxidant and antimicrobial agent because it contains bioactive compounds. This research was conducted to optimize the yield of extract with variations of time and ratio of ethanol as solvent. Optimization time and ratio of solvent for extraction were analyzed using the Response Surface Methodology (RSM) with the Central Composite Design (CCD) model. Extraction of Syringodium isoetifolium was carried out using the Ultrasound Assisted Extraction (UAE) method. The duration of extraction were 2 minutes, 5 minutes, 8 minutes, while the ratio of solvent were 1:10, 1:20, 1:30. Then, these variations were optimized to determine the optimum conditions for the yield of extraction. As result, the optimum condition producing a yield of 14.021% of Syringodium isoetifolium extract was 5.549 minutes with 1:30 ratio of solvent. Meanwhile, Syringodium isoetifolium extract at optimum conditions has an antioxidant activity of 3654.24 ppm and a total phenolic content of 8.05 mgGAE/g.
Keywords: Antioxidant Activity, Syringodium isoetifolium, Total Phenolic Content, Ultrasonic, Yield
