Optimization of Macroaggregated Albumin (MAA) kit Formulation for Lung Perfusion Imaging Wening Lestari, Sulaiman, Puji Widayati, Amal Rezka Putra, Ahsanal Fikri, Sumandi Juliyanto, and Veronika Yulianti Susilo
Badan Riset dan Inovasi Nasional
Abstract
Lung cancer patients in Indonesia are increasing, one of the impacts of which is the disruption of lung function as an organ of exchange for carbon dioxide (CO2) and oxygen (O2). Macroaggregated Albumin (MAA) radiopharmaceutical kits provide an important role in terms of diagnosing pulmonary disorder. MAA kit consists of aggregated Human Serum Albumin (HSA) with a size distribution between 10-90 μ-m, and there is no particle larger than 150 μ-m. The particle size in the MAA kit is one of the important parameters and to obtain suitable particles many modifications of the method have been carried out. In this research, the reaction time, stirring speed and pH during manufacturing process of MAA kit were optimized. The particles size was analyzed using a haemocytometer and observed under a microscope. Optimization of pH was carried out by adding the amount of ascorbic acid. Another important parameter of MAA kit is radiochemical purity which analyzed using paper chromatography method. The MAA formulation was carried out under optimum conditions, ie reaction time for 30 minutes while stirring at a speed of 900 rpm. Ascorbic acid added as much as 1.14 M to obtained pH 5-7 and high radiochemical purity.
