|
Total Phenolic Content and Free Radical Scavenging Activity of Tingkem (Bischofia javanica Blume) Leaves Extract Collected from Lawe Gurah Forest, Ketambe a) Department of Chemistry, Faculty of Science and Technology, Samudra University, Langsa, 24416 Indonesia Abstract The leaves of Tingkem (Bischofia javanica Blume) are recognized for containing various secondary metabolites with potential as natural antioxidants. This research aims to determine the total phenolic content and free radical scavenging activity in Tingkem leaves methanol extract (B. javanica Blume) collected from Lawe Gurah Forest, Ketambe. Phytochemical screening revealed the presence of alkaloids, steroids, terpenoids, tannins, flavonoids, and phenolics in the methanol extract, each contributing to its biological activity. The total phenolic content was measured using the Gallic Acid Equivalent (GAE) standard, yielding 2.152 mg GAE/g extract. Additionally, antioxidant activity was assessed through the DPPH radical scavenging method, establishing the IC50 value as an indicator of antioxidant potency. Results showed that Tingkem leaves methanol extract had an IC50 of 50.139 ppm, suggesting strong antioxidant properties. These findings suggest that Tingkem leaves extract could serve as a valuable natural antioxidant source, aiding in the reduction of oxidative stress and contributing to health preservation through the prevention of degenerative diseases. Based on the phenolic content and substantial free radical scavenging ability, this extract shows promise for future applications in the pharmaceutical and health sectors as a plant-derived antioxidant. This study encourages further investigation into the active metabolites and toxicity profiling to support the broader use of Tingkem as a source of valuable phytochemicals. Keywords: Antioxidants- Phenolics- Bischofia javanica Blume- Tingkem- Ketambe. Topic: Applied Physics and Chemistry |
| SIC 2024 Conference | Conference Management System |