The Effects of Torbangun Leaf Extract (Plectranthus amboinicus) on the Inhibition Zone of Fusobacterium nucleatum and Treponema denticola Growth
Rezky Anggraeni,1 Jessica Endriyana,1*Alfred Pakpahan,1 Juniarta Manalu,2 Aura Sabita2

Faculty of Dentistry Universitas Trisakti

Abstract

Background: Natural ingredients are an alternative that can be chosen as antibacterial agents. P. amboinicus leaves are plants used by some Indonesian people as herbal medicine. Periodontitis is an inflammatory disease in the oral cavity that is widely experienced by the Indonesian population. Bacteria in subgingival plaque that are the main cause of periodontal disease include Fusobacterium nucleatum and Treponema denticola. In addition to periodontal therapeutic therapy, preventive care is also needed, which involves plaque control by brushing teeth and using mouthwash. A natural ingredient that can be used is the P. amboinicus plant. Objectives: To determine the diameter of the growth inhibition zone of Fusobacterium nucleatum and Treponema denticola. Methods: The antibacterial activity was carried out using the agar well diffusion method and chlorhexidine as positive control. Fusobacterium nucleatum and Treponema denticola bacteria were treated with ethanol, ethyl acetate, and hexane fractions of P. amboinicus leaf extract at concentrations of 25%, 50%, and 75% for 24 hours. Results: Treatment of ethanol extract of P. amboinicus leaves, Hexane Fraction and 75% ethanol, effectively Inhibits T. denticola and F. nucleatum with inhibition zones of 13.93 mm and 14.68 mm, respectively, which is significantly different from the negative control. Conclusions: Ethanol and hexane fractions of P. amboinicus leaf extract have inhibitory effects on the growth of F. nucleatum and T. denticola bacteria.

Keywords: Plectranthus amboinicus, Fusobacterium nucleatum, Treponema palidum

Topic: Biology and Microbiology