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Compressive Strength and Swelling Ability of Cube Scaffold Hydroxyapatite With Amniotic Membrane (A Pilot Study) 1. Department of Dental Material Faculty Of Dentistry Universitas Trisakti, Jakarta, Indonesia Abstract Regeneration of large bone defects requires material to stimulate new bone formation. Combining hydroxyapatite cube with amniotic membrane is expected to be a scaffold to replace large bone defects, and accelerate the formation of new bone. This research aims to analyze combined cube scaffold hydroxyapatite with amniotic membrane to compressive strength and swelling ability. Cubes of hydroxyapatite were prepared and immersed in fresh amnion membrane slurry for 5 minutes. After the amnion membrane slurry was fully absorbed by hydroxyapatite, the cubes were frozen for 24 hours at minus 80 degrees Celsius and dried frozen for 24 hours at minus 100 degrees Celsius. After the scaffold cube was fabricated, compressive strength and swelling ability were tested in 10 samples each. The compression strength test was carried out with UTM by applying a force of 50 kgf with a CHS of 0.5 mm per second. Immersed time for the swelling test with NaCl solution was carried out at 1, 7, 14, 21, and 28 days. The mean value of the compressive strength cube scaffold is 2.72 MPa. Meanwhile, the mean values of the swelling ability percentage on days 1, 7, 21, and 28 in consecutive 78.75, 86.40, 89.99, 94.83, and 100.00 showed increased swelling ability. The statistical analysis of swelling ability with repeated ANOVA post hoc Bonferroni was significantly different. Conclusion of the research, the mean value of compressive strength and swelling ability of cube hydroxyapatite with amnion membrane shows potential as a scaffold for a bone defect. Keywords: Amniotic Membrane, Compressive Strength, Hydroxyapatite, Swelling Ability, Scaffold Topic: Biomaterial and Technology in Dentistry |
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