Demineralized Dentin Matrix (DDM) of Human Teeth Can Increase Fibroblasts Cells and Density of Type I Collagen After Tooth Extraction
Renie Kumala Dewi(1,2), Sri Oktawati(3*), Asdar Gani(4), Eko Suhartono(5), Nurlinda Hamrun(6), Rajendran Ganesh(7), Nadira Sapphira(8), Irham Taufiqurrahman(9)

1)Doctoral Program, Faculty of Dentistry, Hasanuddin University, 90245, Makassar, Indonesia
2)Department of Pediatric Dentistry, Faculty of Dentistry, Lambung Mangkurat University, 70123, Banjarmasin, Indonesia
3*,4) Department of Periodontology, Faculty of Dentistry, Hasanuddin University, 90245, Makassar, Indonesia
5) Department of Medical Chemistry and Biochemistry, Faculty of Medicine, Lambung Mangkurat University, 70123, Banjarmasin, Indonesia
6) Department of Oral Biology, Faculty of Dentistry, Hasanuddin University, Makassar, Indonesia
7) Rajendran Ganesh, Assistant Professor of Pediatric Dentistry, Department of Preventive Dental Sciences, Faculty of Dentistry Al Baha University, Al Baha, Saudi Arabia.
8) Postgraduated Student of the Faculty of Dentistry, Lambung Mangkurat University, 70122, Banjarmasin, Indonesia
9) Department of Oral Maxillofacial Surgery, Faculty of Dentistry, Lambung Mangkurat University, 70123, Banjarmasin, Indonesia
*periounhas_sri[at]unhas.ac.id

Abstract

Background: Bone grafts can be used to increase bone regeneration, one of which is allograft. A human tooth graft is classified as an allograft because it is still in the same species. Allogenic Demineralized Dentin Matrix (DDM) is derived from the dentin of human teeth. The composition of dentin is so similar to bone that it is thought to have osteoconductivity.
Purpose: Evaluating the effect of DDM gel on the expression of the number of fibroblast cells and the density of type 1 collagen.
Methods: The left mandibular incisive tooth guinea pig is extracted, then the socket is filled with a Polyethylene glycol gel as a placebo in the control group (n=9), the treatment group (n=9) is filled with DDM gel. The gel is inserted into the socket until it is full then suture with non-absorbable silk. The guinea pig in euthanasia and seen the number of fibroblast cells and the density of type 1 collagen on the 7th, 14th, and 21st days. The research results of the number of fibroblasts using Oneway ANOVA (p<0.05) and type I collagen density were analyzed using the Kruskal Wallis test (p<0.05).
Result: There is a significant difference between the treatment group and control with the significance value in the average density of type 1 collagen (0.009) and the fibroblast cells (0.001).
Conclusion: DDM has the effect of increasing fibroblast cells and density of type I collagen on the -7th, -14th and 21st days, and might be associated with the increase of bone remodeling.

Keywords: Bone graft, Demineralized Dentin Matrix (DDM), Fibroblast, Type I Collagen

Topic: Regenerative Medicine