The potential of Spatholobus littoralis Hassk. Stem Extract as a Local Hemostatic Agent on Sprague Dawley Rats
Juan Arthur Gilberto Silimalar(1) Yessy Ariesanti(2*), Wiwiek Poedjiastoeti(2), Anggraeny Putri Sekar Palupi(2), Irvan Septrian Syah Putra Rasad(2), Komariah Komariah(3)

1Bachelor Program, Faculty of Dentistry, Trisakti University, Jakarta, Indonesia
2Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia
3Department of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia

Abstract

Background: Tooth extraction can cause bleeding complications. The body^s response to stop bleeding is known as hemostasis, which can be accelerated using external material. S. littoralis contains active compounds that in other plants can accelerate hemostasis activity. Objective: This research aimed to evaluate the potential of S. littoralis stem extract as a local hemostatic agent. Method: In vivo and in vitro laboratory experiments were conducted on 18 Sprague Dawley rats divided into six treatment groups: negative control (no treatment), positive control (tranexamic acid), S. littoralis stem extract with concentrations of 25%, 50%, 75%, and 100%. Result: The results of this study showed that S. littoralis stem extract with concentrations of 50%, 75%, and 100% was proven to have the ability to accelerate bleeding time (BT), prothrombin time (PT), and activated partial thromboplastin time (aPTT). The highest concentration, 100%, had the fastest local hemostatic activity, with an average of 25 seconds in the BT test, 20 seconds in the PT test, and 17 seconds in the aPTT test. The study data was found to be normally distributed and homogeneous (p>0.05) through normality and homogeneity testing. ANOVA One Way test revealed significant differences between the treatment groups in the BT, PT, and aPTT tests (p<0.05). The post hoc test confirmed that the 100% extract was as effective as the positive control, 500mg/mL tranexamic acid. Conclusion: S. littoralis stem extract has the potential to be a local hemostatic agent and 100% concentration was found to be as effective as 500mg/5mL tranexamic acid.

Keywords: activated partial thromboplastin time- bleeding time- hemostasis- prothrombin time- Spatholobus littoralis Hassk

Topic: Oral and Maxillofacial Surgery