Vitamin D Inhibits Periodontitis By Lowering The RANKL/OPG Ratio in Wistar Rats
Hervina(a*), I Made Bakta(b), I Nyoman Mantik Astawa(c), Luh Putu Ratna Sundari(d)

a) Department of Periodontic, Faculty of Dentistry, Universitas Mahasaraswati Denpasar, Indonesia
* hervina.drg[at]unmas.ac.id
b) Department of Internal Medicine, Faculty of Medicine, Universitas Udayana, Indonesia
c) Department of Immunology, Faculty of Veterinary, Universitas Udayana, Indonesia
d) Department of Physiology, Faculty of Medicine, Universitas Udayana, Indonesia

Abstract

Background: The modulation of osteoclast-mediated alveolar bone resorption in periodontitis is determined by the relative levels of Receptor Activator of Nuclear Factor-Kappa &#946- Ligand (RANKL) and its decoy receptor, osteoprotegerin (OPG). An increased RANKL/OPG ratio has been reported in periodontitis. Previous observational studies found that periodontitis sufferers had lower serum vitamin D levels. Objective: To evaluate vitamin D^s effect in inhibiting periodontitis by lowering the RANKL/OPG ratio. Methods: The research is an experimental randomized post-test only control group design using 27 Wistar Rats divided into 3 groups where Group I: healthy rats were given 2000IU vitamin D, group II: rats induced periodontitis with Pg bacteria and not given vitamin D were only given regular food, and Group III: rats induced periodontitis with Pg bacteria and given vitamin D 2000IU and given regular food. Each group was observed on the 7th, 14th, and 28th day. Serum RANKL and OPG levels were measured using Enzyme-linked immunosorbent assays, which were analyzed using the One-Way Anova and post hoc tests with LSD. Results: There were differences in the RANKL/OPG ratio that occurred on day 14th (p= 0,011) and 28th (p=0,005). The RANKL/OPG ratio was lower in the Group III (6,70+1,13) than in the Group II (13,95+0,92) on day 14th (p = 0,005) and also lower in the Group III (6,94 + 3,21) compared to the Group II (26,10 + 8,60) at day 28th (p= 0,005). Conclusion: Vitamin D inhibits periodontitis in Wistar rats by lowering the RANKL/OPG ratio starting on day 14th

Keywords: Vitamin D, RANKL/OPG ratio, Periodontitis, Wistar Rat

Topic: Biomedical and Health Informatics