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ANTIBACTERIAL EFFICACY OF REFLUX AND MACERATION EXTRACTS OF PEPEROMIA PELLUCIDA (L.) KUNTH AGAINST PORPHYROMONAS GINGIVALIS (LABORATORY STUDY FOR ADJUNCTIVE THERAPY OF CHRONIC PERIODONTITIS) Fakultas Kedokteran Gigi Universitas Padjadjaran Abstract Porphyromonas gingivalis is a key pathogenic bacterium involved in the development of chronic periodontitis, found in 85.75% of subgingival plaque. The extract of pepper elder leaves (Peperomia pellucida (L.) Kunth) contains natural compounds known for their antibacterial, anti-inflammatory, and antioxidant properties, which may potentially counteract periodontitis. Maceration and reflux are simple natural extraction methods, where reflux was conducted with heating and required less time. This study aimed to determine the difference in antibacterial efficacy between maceration and reflux extracts of pepper elder against Porphyromonas gingivalis. This experimental laboratory study used a Post-Test Control Group Design. Testing was done using the disk diffusion method on 10 treatment groups: maceration and reflux methods with concentrations of 25%, 50%, 75%, 100%, negative control with distilled water, and positive control with 0.2% chlorhexidine. Each treatment group was repeated three times on blood agar media incubated for 48 hours. Measurements were made using calipers on the clear zones formed around the disk paper. The Kruskal-Wallis test showed a p-value of 0.001, indicating significant differences among the groups. The Mann-Whitney test for two-group comparisons revealed significant values, with the highest inhibitory effect observed in the 100% maceration extract. The study found significant differences in the antibacterial efficacy of maceration and reflux extracts against the growth of Porphyromonas gingivalis. Keywords: Reflux, Maceration, Pepper Elder Leaves, Peperomia pellucida (L.) Kunth, Porphyromonas gingivalis. Topic: Periodontics |
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