Inhibition of HSC-3 Cells Migration by Borassus flabellifer L. Seed Coat Ethanol Extract
Pretty Trisfilha, Janti Sudiono, Namita Prayorina Nugroho

Department of Oral & Maxillofacial Pathology, Faculty of Dentistry, Universitas Trisakti, Jakarta, Indonesia

Abstract

One of Indias summer fruits, Borassus flabellifer L. (B.flabellifer L.) has a good source of bio active compounds that make it a potentially useful medicinal herbal. Objectives : The purpose of this study was to identify the bio active compounds of B.flabellifer L. seed coat ethanol extract and investigate its ability to inhibit migration of human oral squamous carcinoma3 (HSC3) cells. Methods : Total phenolic compounds and total flavonoids in B.flabellifer. L seed coat ethanolic extract were determined by colorimetric assay. This in-vitro experimental study was conducted with seven study groups. The extract group was divided into five concentration93,75- 187,5- 375- 750- and 1500 microgram/mL, with doxorubicin (DXR) as positive control and 0.1percent DMSO in culture media as negative control. Wound healing assay were carried out to assess the effect of B.flabellifer L. seed coat ethanol extract on HSC3 cells migration, with percent wound closure as indicator of cell migration. Results : The colorimetric analysis revealed the presence of phenolic, flavonoid, terpenoid, tannin, and alkaloid in B.flabellifer L. seed coat ethanol extract. The extract was able to inhibit migration of HSC3 cells. At 750 microgram/mL, the extract demonstrated the smallest wound closure among other concentrations. Conclusion : This study shows that B.flabellifer L. seed coat ethanol extract exerts anticancer activities in OSCC by inhibiting cell migration. The best results are at a concentration of 750 microgram/mL.

Keywords: Borassus flabellifer. L, HSC-3 cell, migration, wound healing assay

Topic: Cancer Medicine