Malondialdehyde Levels Increased in Male Wistar Rats Induced with Porphyromonas gingivalis Bacteria
Ni Wayan Arni Sardi(a), I Wayan Putu SutirtaYasa(b), Dewa Made Wedagama(c)

a. Faculty of Dentistry - Universitas Mahasaraswati Denpasar, b. Faculty of Medicine - Udayana University,
c. Faculty of Dentistry - Universitas Mahasaraswati Denpasar

Abstract

Backgrounds: Several recent studies indicate an intricate relationship between oxidative stress and inflammation in periodontal tissue. An indicator of oxidative stress is Malondialdehyde (MDA), formed as a result of lipid peroxidation which were pivotal mechanism that were found in cell membranes, leading to the damage and disruption of cellular structures. Objectives: The aim of this study was to compare the MDA levels in the blood plasma of Wistar rats induced with bacteria with those of healthy ones. Methods: This study used post-test only control group design. One group of 4 male wistar rats that induced with Pophyromonas gingivalis and one group of 4 healthy male wistar rats without bacteria induction. The plasma blood was taken from the infraorbital after a week of bacterial induction. ELISA was used to examine the levels of MDA in the plasma. Results: The result showed that there was significant difference level of MDA in the mean score. The mean score of MDA in healthy rats detected 0.300 nmol/mL and MDA level in induced rats found 1.550 nmol/mL. Discussions : Lipopolysacharrides (LPS) from P. gingivalis in sulcus gingiva induced inflammation and induced immune respond that increased neutrophils and macrophage which increased the amount of reactive oxigen species that leads to lipid peroxidation of polyunsaturated fatty acids that produce MDA. Conclusion: higher levels of MDA detected in the group of wistar rats that induced with bacteria compare to the healthy rats.

Keywords: Malondialdehyde, oxidative stress, periodontal tissue, Porphyromonas gingivalis

Topic: Periodontics