Preventive Antibiofilm Activity of Rosella Petals Mouthwash Formulation on Aggregatibacter actinomycetemcomitans: in vitro study
Mikha Sundjojo*, Trijani Suwandi*, Caesary Cloudya Panjaitan**

*Department of Periodontics, Universitas Trisakti, Indonesia
**Department of Public Health and Preventive Dentistry, Universitas Trisakti, Indonesia

Abstract

Background(s): Based on basic health research data in Indonesia, the incidence of periodontitis in the general population was 74.1% in 2018. Aggregatibacter actinomycetemcomitans is dominant in periodontitis molar incisor pattern. Preventing recurrence of periodontitis recommendations are mechanical plaque removal and adjunctive antiseptic mouthwash. Rosella petals contains antimicrobial effect and have potential as alternative mouthwash for periodontitis.
Objective(s): The aim of this study was to analyze the preventive antibiofilm activity of rosella petals mouthwash formulation on Aggregatibacter actinomycetemcomitans in vitro.
Method(s): Phytochemical tests were done to identify the rosella petals mouthwash formulation active substances. Furthermore, a crystal violet biofilm assay was used to analyze biofilm growth with different concentrations (1.56%, 3.125%, 6.25%, and 12.5%) of rosella petals mouthwash formulation. Biofilm turbidity was measured at 490-nm wavelength using a microplate reader.
Result(s): The results showed that the rosella petals mouthwash formulation contains active compounds of flavonoids, saponins, tannins, and polifenol. The biofilm assay results showed that all rosella concentrations exhibited preventive antibiofilm activity to A. actinomycetemcomitans compared with a negative control (p <0.05).
Conclusion(s): Rosella petals mouthwash formulation were effective in inhibiting biofilm to A. actinomycetemcomitans. Rosella petals mouthwash formulation might be useful as an adjunctive therapy for periodontal supportive care. Future studies are still needed to confirm this result in vivo to analyze the efficacy of rosella petal mouthwash formulation.

Keywords: preventive, antibiofilm, rosella petals, periodontitis, Aggregatibacter actinomycetemcomitans

Topic: Periodontics