Optimization Studies of Lactic Acid Production from Unused Biomass by Microorganisms
Elya Mufidah1*, Mamoru Wakayama2

1 Bioprocess Engineering, Faculty of Agricultural Technology, Universitas Brawijaya, Veteran Street, 65145, Malang, East Java, Indonesia
2 Dept. of Biotechnology, Graduate School of Life Sciences, Ritsumeikan University, 1-1-1 Nojihigashi, Kusatsu, Shiga 525- 8577, Japan
Email correspondence: elyamufidah1985[at]ub.ac.id

Abstract

This study divided into two main goals, the optimization of D and L lactic acid production. Optimization of these component factors was performed using the Taguchi method with an L8 orthogonal array. The first goal this study investigated optimization of D lactic acid production from unutilized biomass, specifically banana peel and corncob by multiple parallel fermentation (MPF) with Leuconostoc mesenteroides and Aspergillus awamori. The factors involved in MPF that were assessed in this study comprised banana peel and corncob, KH2PO4, Tween 80, MgSO4 7H2O, NaCl, yeast extract, and diammonium hydrogen citrate to identify the optimal concentration for D lactic acid production. The second goal this study investigated optimization of L lactic acid production from banana peel by multiple parallel fermentation (MPF) using Bacillus licheniformis and Aspergillus awamori. The factors studied in this study consist of banana peel, potassium dihydrogen phosphate, Tween 80, magnesium sulfate heptahydrate, sodium chloride, yeast extract, and ammonium sulfate for optimization of L lactic acid production. Thus, both banana peel and corncob are unutilized potential resources for lactic acid production. The result indicates that optimization of MPF could constitute part of a potential industrial application of the currently unutilized biomass.

Keywords: Optimization- Lactic Acid- Unused biomass- Microbial

Topic: Food biotechnology and bioprocess engineering