Utilization of Tofu Dregs as a Carbon Source in Alternative Growth Mediums of Lactobacillus plantarum with Concentration Factors of Tofu Dregs Flour
Sakunda Anggarini, Suprayogi, Sinta Rahma Fandini

Universitas Brawijaya

Abstract

Lactobacillus plantarum (L. plantarum) is a lactic acid bacterium that has the ability to produce lactic acid and is widely used in industry. An important factor in bacterial growth is the selection of a good and inexpensive medium. Tofu dregs are solid waste produced by the tofu industry and have the potential to be used as a carbon source in L. plantarum alternative medium as an energy source in growing and producing products. The results showed that tofu dregs could potentially be used as an alternative medium carbon source for L. plantarum due to the relatively high carbohydrate content of 73.17%. In the growth test, it was found that the treatment medium had an increase in OD600 and TPC almost the same as the control medium, and the specific growth rate was higher than the control medium. The pH value of the treatment medium showed a decrease, which meant that L. plantarum during the incubation period produced a metabolite product in the form of lactic acid, supported by data on an increase in TAT. So it was concluded that the use of tofu dregs as an alternative medium supports the growth of L. plantarum but does not produce high lactic acid products and the tofu dregs medium with the best concentration is in the 10% w/v treatment with an OD600 value of 1.30 and a TPC value of 1.54 log CFU/mL with a growth rate of 0.36h-1.

Keywords: alternative medium, carbon source, Lactobacillus plantarum, tofu dregs

Topic: Waste and environmental management