Isolation Of Cellulose-Producing Bacteria (K. Saccharivorans) From Rotten Sapodilla Fruit
Claudia Gadizza Perdani a) Irnia Nurika b) Setiyo Gunawan a*)

a) Department of chemical engineering Institut Teknologi Sepuluh Nopember Surabaya
b) Department of agroindustrial technology Faculty of agricultural technology Universitas Brawijaya Malang
gunawan[at]chem-eng.its.ac.id

Abstract

Bacterial nano cellulose (BNC) is a nano cellulose produced by bacteria with high purity, crystallinity level, water binding ability, a high degree of polymerization, and excellent mechanical characteristics. The selection of substrate and BNC-producing bacteria is one of the critical stages in the production of BNC. In this study, we successfully isolated acetic acid bacteria that potentially produce high-value BNC from rotten sapodilla fruit. Based on the 16s rRNA strain analysis, sapodilla isolate has high similarities with the bacteria Komagataeibacter saccharivorans. The yield of BNC-produced isolate sapodilla Komagataeibacter saccharivorans 0.432 g/L was mainly determined as cellulose I (adsorption at around 3345, 1430, 1160, and 900 cm-1), few celluloses II (adsorption at around 1335, 1315, and 1280 cm-1 and a blue-shift of the number of waves from 1430 to around 1425 cm-1). The crystallinity index of BNCs obtained (58%) in this study was lower than standard BNC produced by Komagataeibacter xylinus (80%).

Keywords: BNC, nanocellulose, packaging, sustainable

Topic: Food biotechnology and bioprocess engineering