THE EFFECT OF NAA AND BAP IN INDUCTION OF PROTOCORM LIKE BODIES (PLB) Cattleya sp. ORCHID IN VITRO Anita Rasuna Sari Siregar1, Fauziyah Harahap2, Idramsa3, Emmy Harso Kardhinata4, Armaniar5
Postgraduate Student of Biology Education Program, Universitas Negeri Medan Jalan Willem Iskandar/Pasar V, Medan 2023, Indonesia1, Lecture of Biology Education Program, Universitas Negeri Medan Jalan Willem Iskandar/Pasar V, Medan 2023, Indonesia2,3, Lecture of Agroecotechnology Study Program,Universitas Negeri Medan, Medan 2023 ,Indonesia4, Lecture of Agroecotechnology Study Program, Universitas Panca Budi, Medan, Indonesia5
Abstract
Orchid is a type of ornamental plant whose existence in the wild has decreased and is threatened with extinction, orchid has high aesthetic value and economic value.Cattleya orchids generally have larger flower sizes compared to other orchids, so Cattleya is nicknamed The Queen of Orchid, Cattleya sp. seeds do not have an endosperm as a food reserve, so they need nutrients to germinate to help seed growth. To preserve this plant, it needs to in vitro technology. Efforts to propagate plants using in vitro techniques (tissue culture) on selected cultivars by forming Protocorm-Like Body (PLB) or somatic embryos through somatic embryogenesis is a method capable of producing large quantities of orchid seeds quickly. The aim of this research was to determine the best interaction of growth regulator naphthalene acetic acid (NAA), benzyl amino purine (BAP) and interaction of both on growth induction of Protocorm-Like Body of Cattleya sp. The research method used a completely random design with various concentrations of naphthalene acetic acid (NAA: 0- 0.5- 1- 1.5 ppm) and benzyl amino purine concentration (BAP: 0- 0,5- 1 ppm). Parameters of observation of PLB formation time directly from explants, number of PLB and PLB color. The data were analyzed descriptively and inferentially with ANAVA and color observation using the colorgrab application. The result showed there is significantly effect of NAA and BAP interaction on the time PLB was formed and there is no significant interaction on the number of PLB. PLB formed the fastest in week 3 with the media treatment MS + NAA 1.5 ppm + BAP 1 ppm, the highest number of PLB was found in MS + NAA 1.5 ppm + BAP 1 ppm which was 6,67 PLB. 3 PLB color variations were produced, with the best color being green.
Keywords: Protocorm-Like Bodies , Cattleya sp. , In vitro
