Effect of regulating the silver nitrate solution on the growth of honey-mediated silver nanoparticles via green synthesis for antibacterial application
Aaron Andrew B. Mutia (a,b,f*), Rey Marc T. Cumba (d), Archie G. Ruda (a,b), Marjune T. Bonilla (a,b), Kurt Sterling M. Ubas (a,b), Lloyd Allan T. Cabanog (a,b,f), Rey Y. Capangpangan (c), Joselito P. Labis (e), Arnold C. Alguno (a,b)

a)Department of Physics, College of Science and Mathematics, MSU-IIT, 9200 Iligan City Philippines
b) Premier Research of Science and Mathematics (PRISM), Mindanao State University - Iligan Institute of Technology, 9200 Iligan City, Philippines
c) Department of Physical Sciences and Mathematics, Mindanao State University - Naawan Campus, Naawan Misamis Oriental
d) Department of Chemistry, Caraga State University, Ampayon, Butuan City, 8600 Agusan del Norte Philippines
e) King Abdullah Institute of Nanotechnology, Riyadh 11451, Saudi Arabia
f) Department of Physics, College of Arts and Sciences, Central Mindanao University, Maramag 8714, Bukidnon, Philippines

*aaaronandrew.mutia[at]g.msuiit.edu.ph

Abstract

Silver nanoparticles were synthesized using locally purchased honey and silver nitrate solution. This method provides a simplistic and straightforward approach to the formation of silver nanoparticles. The silver nanoparticles with varying amounts of silver nitrate solution were characterized using ultraviolet-visible spectroscopy and Fourier transform infrared spectroscopy. In addition, dynamic light scattering characterization was used to determine the average size and size distribution of silver nanoparticles. Experimental results revealed that varying the amount of silver nitrate solution can control the size and absorption spectra of silver nanoparticles. A large amount of silver nitrate solution will exhibit a peak in the higher wavelength. The shifting of the SPR bands are believed to be related to the surface plasmon resonance of nanoparticles. Moreover, a larger amount of silver nitrate solution also results in an increasing hydrodynamic size with 27.2, 57.9, and 63.4 nm as revealed in the size distribution via dynamic light scattering which also coincides with the particle size using TEM image analysis with 12.247 nm, 14.644 nm, and 45.234 nm. The synthesized silver nanoparticles have been found to be a face-centered cubic crystal structure consisting of peaks of (111), (200), (220), and (311) planes with an average size of ~10.88 nm. This green synthesis method of silver nanoparticles will provide a low cost-effective production as an alternative to commercial antibacterial agents.

Keywords: silver nanoparticles, green synthesis, honey, UV-Vis spectroscopy, Dynamic light scattering, Fourier Transform Infrared spectroscopy, surface plasmon resonance, Transmission Electron Microscope, X-ray Diffraction

Topic: Material and Applied Chemistry